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Exploring the Apoptotic-Induced Biochemical Mechanism of Traditional Thai Herb (Kerra?) Extract in HCT116 Cells Using a Label-Free Proteomics Approach
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Document Title
Exploring the Apoptotic-Induced Biochemical Mechanism of Traditional Thai Herb (Kerra?) Extract in HCT116 Cells Using a Label-Free Proteomics Approach
Author
Siriwaseree J. Yingchutrakul Y. Samutrtai P. Aonbangkhen C. Srathong P. Krobthong S. Choowongkomon K.
Affiliations
Department of Biochemistry Faculty of Science Kasetsart University Bangkok 10900 Thailand; National Center for Genetic Engineering and Biotechnology NSTDA Pathum Thani 12120 Thailand; Department of Pharmaceutical Sciences Faculty of Pharmacy Chiang Mai University Chiang Mai 50200 Thailand; Center of Excellence in Natural Products Chemistry (CENP) Department of Chemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Faculty of Nursing Praboromarajchanok Institute Nonthaburi 11000 Thailand; Interdisciplinary Graduate Program in Genetic Engineering Kasetsart University Bangkok 10900 Thailand
Type
Article
Source Title
Medicina (Lithuania)
ISSN
1010660X
Year
2023
Volume
59
Issue
8
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/medicina59081376
Abstract
Background and Objectives: Natural products have proven to be a valuable source for the discovery of new candidate drugs for cancer treatment. This study aims to investigate the potential therapeutic effects of 揔erra?� a natural extract derived from a mixture of nine medicinal plants mentioned in the ancient Thai scripture named the Takxila Scripture on HCT116 cells. Materials and Methods: In this study the effect of the Kerra? extract on cancer cells was assessed through cell viability assays. Apoptotic activity was evaluated by examining the apoptosis characteristic features. A proteomics analysis was conducted to identify proteins and pathways associated with the extract抯 mechanism of action. The expression levels of apoptotic protein markers were measured to validate the extract抯 efficacy. Results: The Kerra? extract demonstrated a dose-dependent inhibitory effect on the cells with higher concentrations leading to decreased cell viability. Treatment with the extract for 72 h induced characteristic features of early and late apoptosis as well as cell death. An LC-MS/MS analysis identified a total of 3406 proteins. The pathway analysis revealed that the Kerra? extract stimulated apoptosis and cell death in colorectal cancer cell lines and suppressed cell proliferation in adenocarcinoma cell lines through the EIF2 signaling pathway. Upstream regulatory proteins including cyclin-dependent kinase inhibitor 1A (CDKN1A) and MYC proto-oncogene bHLH transcription factor (MYC) were identified. The expressions of caspase-8 and caspase-9 were significantly elevated by the Kerra? extract compared to the chemotherapy drug Doxorubicin (Dox). Conclusions: These findings provide strong evidence for the ability of the Kerra? extract to induce apoptosis in HCT116 colon cancer cells. The extract抯 efficacy was demonstrated by its dose-dependent inhibitory effect induction of apoptotic activity and modulation of key proteins involved in cell death and proliferation pathways. This study highlights the potential of Kerra? as a promising therapeutic agent in cancer treatment. ? 2023 by the authors.
Keyword
caspase-8 | caspase-9 | CDKN1A | colorectal cancer | LC-MS/MS | MYC | traditional herbs
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS