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Fluoride Concentration Antibacterial Effect and Cytotoxicity in Children s Toothpaste In Vitro Study
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Document Title
Fluoride Concentration Antibacterial Effect and Cytotoxicity in Children s Toothpaste In Vitro Study
Author
Thongthai P. Sivavong P. Osathanon T. Tantilertanant Y. Sakoolnamarka R. Singthong T. Uttasen S. Boonsuth B. Monmaturapoj N. Nantanapiboon D.
Affiliations
Department of Operative Dentistry Faculty of Dentistry Chulalongkorn University Bangkok Thailand; Dental Stem Cell Biology Research Unit Department of Anatomy Faculty of Dentistry Chulalongkorn University Bangkok Thailand; Dental Material Research and Development Center Faculty of Dentistry Chulalongkorn University Bangkok Thailand; CU Dental Innovation Center Faculty of Dentistry Chulalongkorn University Bangkok Thailand; Oral Biology Research Center Faculty of Dentistry Chulalongkorn University Bangkok Thailand; Assistive Technology and Medical Devices Research Center (A-MED) National Science and Technology Development Agency Pathum Thani Thailand
Type
Article
Source Title
European Journal of General Dentistry
ISSN
22789626
Year
2023
Volume
12
Issue
3
Page
199-208
Open Access
All Open Access Hybrid Gold Green
Publisher
Thieme Medical Publishers Inc.
DOI
10.1055/s-0043-1775829
Abstract
Objectives The aim of this study is to evaluate the effect on cell viability antibacterial activity against cariogenic bacteria and total fluoride availability of commercially available children s toothpastes in Thailand. Materials and Methods Seven toothpaste slurries were prepared from seven commercial toothpastes for children. Total fluoride concentration was determined. The agar diffusion method was used to examine the antibacterial effect of toothpaste against Streptococcus mutans. The viability of L929 mouse fibroblasts exposed with different concentrations of each toothpaste slurry was tested by MTT (3-[4 5-dimethylthiazol-2-yl]-2 5 diphenyl tetrazolium bromide) assay. Statistical Analysis One way analysis of variance (ANOVA) and Tukey s honestly significant difference (HSD) tests were used for zone inhibition analysis. Cell viability data were analyzed using Student s t -test. Results Fluoride concentration in fluoride-containing toothpastes ranged from 521.36 to 1 377.83 ppm. Two toothpastes exhibited a similar level of fluoride concentration compared between the product labels. Other toothpastes showed a difference or slight difference in fluoride concentration between the companies information and our data. The zone of inhibition ranged from 0 to 2.08 cm. A significantly higher zone of inhibition was observed in toothpastes with sodium lauryl sulfate. The concentration of toothpaste that reduced cell viability to less than 50% of the control was 0.8 3.1 6.3 6.3 25 and 100% for Fluocaril Deli Fruity Oral-B Junior 6 + Kodomo Kids CUdent Stevia SunStar GUM and Kindee Organic respectively. Conclusion The examined children s toothpastes can inhibit the growth of S. mutans which did not correlate with fluoride concentration. The antibacterial effects could be the results of other ingredients for example sodium lauryl sulfate. ? 2023 Wolters Kluwer Medknow Publications. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Wolters Kluwer Medknow Publications
Publication Source
Scopus