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Cryo-Induced Cellulose-Based Nanogel from Elaeis guineensis for Antibiotic Delivery Platform
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Document Title
Cryo-Induced Cellulose-Based Nanogel from Elaeis guineensis for Antibiotic Delivery Platform
Author
Hajidariyor T. Nuntawad N. Somsaen P. Prukdamrongchai R. Cherdchoo H. Posoknistakul P. Khemthong P. Wanmolee W. Arjfuk P. Pongchaikul P. Laosiripojana N. Wu K.C.W. Sakdaronnarong C.
Affiliations
Department of Chemical Engineering Faculty of Engineering Mahidol University Nakorn Pathom 73170 Thailand; National Nanotechnology Center (NANOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani12120 Thailand; Chakri Naruebodindra Medical Institute Faculty of Medicine Ramathibodi Hospital Mahidol University Samut Prakarn10540 Thailand; The Joint Graduate School of Energy and Environment King Mongkut抯 University of Technology Thonburi Bangkok 10140 Thailand; Department of Chemical Engineering National Taiwan University Taipei 10617 Taiwan; Center of Atomic Initiative for New Materials (AI-MAT) National Taiwan University Taipei 10617 Taiwan; International Graduate Program of Molecular Science and Technology National Taiwan University (NTU-MST) Taipei 10617 Taiwan; Department of Chemical Engineering and Materials Science Yuan Ze University Taoyuan 32003 Taiwan; Yonsei Frontier Lab Yonsei University Seoul 03722 South Korea
Type
Article
Source Title
International Journal of Molecular Sciences
ISSN
16616596
Year
2023
Volume
24
Issue
2
Open Access
All Open Access Gold Green
Publisher
MDPI
DOI
10.3390/ijms24021230
Abstract
Cryo-induced hydrogel from cellulose is a new class of biomaterials for drug delivery cell delivery bone and skin tissue engineering for cell proliferation and regeneration applications. This research aimed to synthesize cryo-induced hydrogel from cellulose and carboxymethyl cellulose (CMC) produced from empty bunch抯 cell wall of Elaeis guineensis. First the experiment was to produce cellulose-rich material using hot-compressed water extraction followed by alkaline delignification and bleaching with H2O2. The obtained bleached EFB cellulose was used as the substrate for CMC and the optimal condition with the highest degree of carboxyl substitution (DS) of 0.75 was achieved when varying NaOH and monochloroacetic acid concentration as well as etherification temperature using fractional factorial design. For cryogelation study hydrogels were synthesized from cellulose CMC and beta-cyclodextrin (?-CD) by dissolving cellulose-based matrix in a NaOH/urea system and the cellulose (CEL) solution was frozen spontaneously at ?40 ?C followed by high speed mixing to loosen cellulose fibrils. Epichlorohydrin (ECH) and Polyethylene glycol diglycidyl ether (PEGDE) were used as a cross-linker. First the ratio of cellulose and CMC with different amounts of ECH was investigated and subsequently the proper ratio was further studied by adding different crosslinkers and matrices i.e. CMC and ?-CD. From the result the ECH crosslinked CMC-CEL (E-CMC-CEL) gel had the highest swelling properties of 5105% with the average pore size of lyophilized hydrogel of 300 ?m. In addition E-CMC-CEL gel had the highest loading and release capability of tetracycline in buffer solution at pH 7.4 and 3.2. At pH 7.4 tetracycline loading and release properties of E-CMC-CEL gel were 65.85 mg g?1 dry hydrogel and 46.48 mg g?1 dry hydrogel (70.6% cumulative release) respectively. However at pH 3.2 the loading and release capabilities of Tetracycline were moderately lower at 16.25 mg g?1 dry hydrogel and 5.06 mg g?1 dry hydrogel respectively. The findings presented that E-CMC-CEL hydrogel was a suitable material for antibiotic tetracycline drug carrying platform providing successful inhibitory effect on Staphylococcus aureus Escherichia coli and Pseudomonas aeruginosa respectively. ? 2023 by the authors.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS