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Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics
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Document Title
Analyzing Predominant Bacterial Species and Potential Short-Chain Fatty Acid-Associated Metabolic Routes in Human Gut Microbiome Using Integrative Metagenomics
Author
Kingkaw A. Raethong N. Patumcharoenpol P. Suratannon N. Nakphaichit M. Keawsompong S. Roytrakul S. Vongsangnak W.
Affiliations
Interdisciplinary Graduate Program in Bioscience Faculty of Science Kasetsart University Bangkok 10900 Thailand; Institute of Nutrition Mahidol University Nakhon Pathom 73170 Thailand; Center of Excellence for Allergy and Clinical Immunology Division of Allergy Immunology and Rheumatology Department of Pediatrics Faculty of Medicine Chulalongkorn University King Chulalongkorn Memorial Hospital The Thai Red Cross Society Bangkok 10330 Thailand; Department of Biotechnology Faculty of Agro-Industry Kasetsart University Bangkok 10900 Thailand; Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency 144 Thailand Science Park Phaholyothin Road Pathum Thani12120 Thailand; Department of Zoology Faculty of Sciences Kasetsart University Bangkok 10900 Thailand; Omics Center for Agriculture Bioresources Food and Health Kasetsart University (OmiKU) Bangkok 10900 Thailand
Type
Article
Source Title
Biology
ISSN
20797737
Year
2023
Volume
12
Issue
1
Open Access
All Open Access Gold Green
Publisher
MDPI
DOI
10.3390/biology12010021
Abstract
Gut microbiome plays an essential role in host health and there is interest in utilizing diet to modulate the composition and function of microbial communities. Copra meal hydrolysate (CMH) is commonly used as a natural additive to enhance health. However the gut microbiome is largely unknown at species level and is associated with metabolic routes involving short-chain fatty acids (SCFAs). In this study we aimed to analyze using integrative metagenomics the predominant species and metabolic routes involved in SCFAs production in the human gut microbiome after treatment with CMH. The effect of CMH treatment on the Thai gut microbiome was demonstrated using 16S rRNA genes with whole-metagenome shotgun (WMGS) sequencing technology. Accordingly these results revealed that CMH has potentially beneficial effects on the gut microbiome. Twelve predominant bacterial species as well as their potential metabolic routes were involved in cooperative microbiome networks under sugar utilization (e.g. glucose mannose or xylose) and energy supply (e.g. NADH and ATP) in relation to SCFAs biosynthesis. These findings suggest that CMH may be used as a potential prebiotic diet for modulating and maintaining the gut microbiome. To our knowledge this is the first study to reveal the predominant bacterial species and metabolic routes in the Thai gut microbiome after treatment with potential prebiotics. ? 2022 by the authors.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS