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High Level of Serum Coiled-coil Domain Containing 25 (CCDC25) as a Diagnostic Marker for Cholangiocarcinoma But Not for Other Cancers
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Document Title
High Level of Serum Coiled-coil Domain Containing 25 (CCDC25) as a Diagnostic Marker for Cholangiocarcinoma But Not for Other Cancers
Author
Jannawan A. Silsirivanit A. Seubwai W. Wongwattanakul M. Proungvitaya T. Aung T.M. Roytrakul S. Chiawiriyabunya I. Proungvitaya S.
Affiliations
Centre of Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand; Cholangiocarcinoma Research Institute Faculty of Medicine Khon Kaen University Khon Kaen Thailand; Department of Biochemistry Center for Translational Medicine Faculty of Medicine Khon Kaen University Khon Kaen Thailand; Department of Forensic Medicine Faculty of Medicine Khon Kaen University Khon Kaen Thailand; Center for Innovation and Standard for Medical Technology and Physical Therapy Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand; Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and Biotechnology Pathumthani Thailand; Udonthani Cancer Hospital Udon Thani Thailand
Type
Article
Source Title
In Vivo
ISSN
0258851X
Year
2023
Volume
37
Issue
1
Page
294-303
Open Access
All Open Access Gold Green
Publisher
International Institute of Anticancer Research
DOI
10.21873/invivo.13079
Abstract
Background/Aim: Recently we reported that coiled-coil domain containing 25 (CCDC25) protein is elevated in the sera of patients with cholangiocarcinoma (CCA) and is suggested to be a diagnostic biomarker for CCA. This study aimed to examine whether serum CCDC25 level can be a unique biomarker for CCA. Bioinformatic analyses using Human Protein Atlas (HPA) database and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) indicated that CCDC25 protein and mRNA are expressed not only in CCA but also in other cancers such as colorectal cancer (CRC) breast cancer (BC) and hepatocellular carcinoma (HCC) all of which are the top 5 cancers highly prevalent in Thailand. Materials and Methods: Using a quantitative dot blot assay serum CCDC25 levels were measured for 30 healthy controls (HC) 34 CRC 42 BC 43 HCC and 83 CCA. Results: The serum CCDC25 levels of CCA patients (0.193?0.039 ng/?l) were significantly higher than those of CRC (0.019?0.006 ng/?l) BC (0.036?0.015 ng/?l) HCC (0.035?0.016 ng/?l) and higher than those of HC (0.012?0.003 ng/?l). The serum CCDC25 level can discriminate CCA from the HC CRC BC and HCC with a sensitivity of 100 99 94 and 94% respectively and specificity of 100 100 98 and 95% respectively. Conclusion: CCDC25 is a candidate diagnostic biomarker for CCA. ? 2023 International Institute of Anticancer Research. All rights reserved.
Keyword
CCA | CCDC25 | diagnostic biomarker | highly prevalent cancers
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-ND
Rights
Authors
Publication Source
WOS