-
Research Note: Possible influence of thermal selection on patterns of HSP70 and HSP90 gene polymorphisms in Thai indigenous and local chicken breeds and red junglefowls
- Back
Document Title
Research Note: Possible influence of thermal selection on patterns of HSP70 and HSP90 gene polymorphisms in Thai indigenous and local chicken breeds and red junglefowls
Author
Budi T., Singchat W., Tanglertpaibul N., Thong T., Panthum T., Noito K., Wattanadilokchatkun P., Jehangir M., Chaiyes A., Wongloet W., Vangnai K., Yokthongwattana C., Sinthuvanich C., Ahmad S.F., Muangmai N., Han K., Nunome M., Supnithi T., Koga A., Duengkae P., Matsuda Y., Srikulnath K.
Affiliations
Doctoral Program in Nutrition, Faculty of Medicine Ramathibodi Hospital, Institute of Nutrition, Mahidol University, Bangkok, 10400, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand; Nutrition Division, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand
Type
Article
Source Title
Nutrients
ISSN
20726643
Year
2024
Volume
16
Issue
6
Open Access
All Open Access, Gold
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/nu16060877
Abstract
Integrated omics-based platforms from epigenomics and proteomics technologies are used to identify several important mechanisms in obesity etiology, food components, dietary intake, regulation of biological pathways, and potential new intervention targets. Therefore, this study aimed to analyze whether dietary factors involved in the methylation of tumor necrosis factor (TNF)-? are implicated in differential protein expression in people with normal weight and obesity. Methods: The participants were classified into the non-obese (N = 100) and obese (N = 133) groups. DNA methylation levels of the TNF-alpha gene and proteomics were analyzed using the pyrosequencing method and LC-MS-MS, respectively. Results: Comparison between geometric means of DNA methylation of TNF-? showed lower levels in subjects with obesity than in those without obesity (p < 0.05). There were associations between dietary factors and some metabolic syndrome components and TNF-? DNA methylation levels. Proteomic analysis showed important signaling pathways related to obesity, with 95 significantly downregulated proteins and 181 upregulated proteins in the non-obese group compared with the obese group. Conclusion: This study shows an association between the dietary factors involved in the methylation of TNF-? and differential protein expression related to obesity. However, a large sample size in future studies is required to confirm our results. ? 2024 by the authors.
License
CC BY
Rights
Authors
Publication Source
WoS