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Enhanced and asymmetric signatures of hybridization at climatic margins: Evidence from closely related dioecious fig species
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Document Title
Enhanced and asymmetric signatures of hybridization at climatic margins: Evidence from closely related dioecious fig species
Author
Huang J.-F., Darwell C.T., Peng Y.-Q.
Affiliations
Faculty of Science, Burapha University, Chonburi, 20131, Thailand; Marine Biotechnology Research Unit, Faculty of Marine Technology, Burapha University, Chanthaburi Campus, Chanthaburi, 22170, Thailand; Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Aquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Faculty of Science and Arts, Burapha University, Chanthaburi Campus, Chanthaburi, 22170, Thailand
Type
Article
Source Title
Aquaculture and Fisheries
ISSN
20961758
Year
2024
Open Access
All Open Access, Gold
Publisher
KeAi Communications Co.
DOI
10.1016/j.aaf.2024.04.011
Abstract
Genes exhibiting differential expression patterns between large-sized (average body weight 17.57 ? 1.8 g and 40.97 ? 4.0 g for 3- and 5-month-old juveniles in a total of 342 and 356 samples, respectively) and small-sized (average body weight 10.01 ? 0.76 g and 22.86 ? 2.51 g, respectively) juvenile giant tiger shrimps (Penaeus monodon) were isolated by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP). In total, 368 primer combinations were screened against the first-strand cDNA of the different groups of 3- and 5-month-old P. monodon. Over 146 differentially expressed or size-specific markers were derived using 59 selective primer combinations. A total of 70 candidate cDNA-AFLP markers—19 size-specific and 51 showing differential expression—were further cloned, sequenced, and blasted against the GenBank database. Twenty-five markers were found to comprise newly identified sequences (E-values >10?4). Quantitative real-time PCR and Single-strand conformational polymorphism (SSCP) analyses of the products generated with the SCAR/cDNA-AFLP primers were further undertaken. Interestingly, the relative expression levels of the products of primers E+34M+314-550-PDI, E+35M+310-350-Unk, E+29/M+211-350-CHK, E+34/M+31-380-phosphotysosyl, and E+38/M+315-800-fucosidase were significantly upregulated in the hepatopancreas of large-sized juvenile shrimp compared with that is small-sized animals. In contrast, the relative expression levels of the products generated by E+31M+38-480-InositolOxy and E+29/M+211-330-vitelline membrane were significantly upregulated in small-sized P. monodon. The consistency of our results was further assessed in 1-month-old aquaculture-raised P. monodon. Single nucleotide polymorphisms were identified using SSCP at different positions in the sequences of fragments of the different groups generated with the primers E+34M+314-550-PDI and E+35M+310-350-Unk. Our results indicated that cDNA-AFLP has the potential for use in the isolation of functionally important transcripts in P. monodon. ? 2024 Shanghai Ocean University.
License
CC BY-NC-ND
Rights
Shanghai Ocean University
Publication Source
Scopus