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Guava and Star gooseberry leaf extracts improve growth performance, innate immunity, intestinal microbial community, and disease resistance in Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
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Document Title
Guava and Star gooseberry leaf extracts improve growth performance, innate immunity, intestinal microbial community, and disease resistance in Nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila
Author
Kamble M.T., Chaiyapechara S., Salin K.R., Bunphimpapha P., Chavan B.R., Bhujel R.C., Medhe S.V., Kettawan A., Thiyajai P., Thompson K.D., Pirarat N.
Affiliations
Center of Excellence in Biodiversity Research, Mahasarakham University, Maha Sarakham, Thailand; Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia; Deparment of Chemistry, College of Science and Engineering, Flinders University, Adelaide, SA, Australia; Department of Genetics, Kasetsart University, Bangkok, Thailand; Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand; Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, Thailand; Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand; National Biobank of Thailand (NBT), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand; Aesthetic Sciences and Health Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubon Ratchathani, Thailand
Type
Article
Source Title
Frontiers in Microbiology
ISSN
1664302X
Year
2024
Volume
15
Open Access
All Open Access, Gold
Publisher
Frontiers Media SA
DOI
10.3389/fmicb.2024.1353511
Abstract
Endophytic actinobacteria are a group of bacteria living inside plant tissue without harmful effects, and benefit the host plant. Many can inhibit plant pathogens and promote plant growth. This study aimed to identify a strain of Streptomyces as a novel species and study its antibiotics production. An endophytic actinobacterium, strain TML10T was isolated from a surface-sterilized leaf of a Thai medicinal plant (Terminalia mucronata Craib and Hutch). As a result of a polyphasic taxonomy study, strain TML10T was identified as a member of the genus Streptomyces. Strain TML10T was an aerobic actinobacterium with well-developed substrate mycelia with loop spore chains and spiny surface. Chemotaxonomic data, including cell wall components, major menaquinones, and major fatty acids, confirmed the affiliation of strain TML10T to the genus Streptomyces. The results of the phylogenetic analysis, including physiological and biochemical studies in combination with a genome comparison study, allowed the genotypic and phenotypic differentiation of strain TML10T and the closest related type strains. The digital DNA-DNA hybridization (dDDH), Average nucleotide identity Blast (ANIb), and ANIMummer (ANIm) values between strain TML10T and the closest type strain, Streptomyces musisoli CH5-8T were 38.8%, 88.5%, and 90.8%, respectively. The name proposed for the new species is Streptomyces naphthomycinicus sp. nov. (TML10T = TBRC 15050T = NRRL B-65638T). Strain TML10T was further studied for liquid and solid-state fermentation of antibiotic production. Solid-state fermentation with cooked rice provided the best conditions for antibiotic production against methicillin-resistant Staphylococcus aureus. The elucidation of the chemical structures from this strain revealed a known antimicrobial agent, naphthomycin A. Mining the genome data of strain TML10T suggested its potential as a producer of antbiotics and other valuable compounds such as ?-Poly-L-lysine (?-PL) and arginine deiminase. Strain TML10T contains the arcA gene encoding arginine deiminase and could degrade arginine in vitro. Copyright ? 2024 Kaewkla, Perkins, Thamchaipenet, Saijuntha, Sukpanoa, Suriyachadkun, Chamroensaksri, Chumroenphat and Franco.
License
CC BY
Rights
Authors
Publication Source
WoS