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Potential Use of Lactiplantibacillus plantarum BCC 4352 as a Functional Starter Culture for Fermenting Thai Pork Sausage (Nham)
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Document Title
Potential Use of Lactiplantibacillus plantarum BCC 4352 as a Functional Starter Culture for Fermenting Thai Pork Sausage (Nham)
Author
Kingcha Y., Pumpuang L., Adunphatcharaphon S., Chantarasakha K., Santiyanont P., Klomtun M., Janyaphisan T., Kongtong K., Phonsatta N., Panya A., Visessanguan W., Petchkongkaew A., Woraprayote W.
Affiliations
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Thailand; Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand; Plant Breeding Research Center for Sustainable Agriculture, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand; Department of Agronomy, Iowa State University, Ames, IA, United States; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand; Rice Science Center, Kasetsart University, Nakhon Pathom, Thailand
Type
Article
Source Title
Frontiers in Plant Science
ISSN
1664462X
Year
2024
Volume
15
Open Access
All Open Access, Gold
Publisher
Frontiers Media SA
DOI
10.3389/fpls.2024.1337463
Abstract
Doubled haploid (DH) technology becomes more routinely applied in maize hybrid breeding. However, some issues in haploid induction and identification persist, requiring resolution to optimize DH production. Our objective was to implement simultaneous marker-assisted selection (MAS) for qhir1 (MTL/ZmPLA1/NLD) and qhir8 (ZmDMP) using TaqMan assay in F2 generation of four BHI306-derived tropical ? temperate inducer families. We also aimed to assess their haploid induction rate (HIR) in the F3 generation as a phenotypic response to MAS. We highlighted remarkable increases in HIR of each inducer family. Genotypes carrying qhir1 and qhir8 exhibited 1 – 3-fold higher haploid frequency than those carrying only qhir1. Additionally, the qhir1 marker was employed for verifying putative haploid seedlings at 7 days after planting. Flow cytometric analysis served as the gold standard test to assess the accuracy of the R1-nj and the qhir1 marker. The qhir1 marker showed high accuracy and may be integrated in multiple haploid identifications at early seedling stage succeeding pre-haploid sorting via R1-nj marker. Copyright ? 2024 Khammona, Dermail, Suriharn, L?bberstedt, Wanchana, Thunnom, Poncheewin, Toojinda, Ruanjaichon and Arikit.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WoS