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Enhanced Stability of Gold Nanoparticles with Thioalkylated Carboxyl-Terminated Ligands for Applications in Biosensing
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Document Title
Enhanced Stability of Gold Nanoparticles with Thioalkylated Carboxyl-Terminated Ligands for Applications in Biosensing
Author
Ngernpimai S., Puangmali T., Kopwitthaya A., Tippayawat P., Chompoosor A., Teerasong S.
Affiliations
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 143 Thailand Science Park, Phaholyothin Rd., Khlong Luang, Pathum Thani, 12120, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Rd., Khlong Luang, Pathum Thani, 12120, Thailand; Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, 114 Sukhumvit 21, Bangkok, 10110, Thailand; National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park (TSP), Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Heliyon
ISSN
24058440
Year
2024
Volume
10
Issue
8
Open Access
All Open Access, Gold
Publisher
Elsevier Ltd
DOI
10.1016/j.heliyon.2024.e29444
Abstract
Fragmentation of ?-glucans secreted by the fungus Ophiocordyceps dipterigena BCC 2073 achieved by microfluidization was investigated. The degree of ?-glucan fragmentation was evaluated based on the average number of chain scissions (?). The effects on the ? value of experimental variables like solid concentration of the ?-glucan suspension, interaction chamber pressure, and number of passes through the microfluidizer were examined. Kinetic studies were conducted using the relationships of the ? and suspension viscosity values with the number of passes. Evidence indicated that ? increases with the interaction chamber pressure and the number of passes, whereas the solid concentration shows the inverted effect. Kinetic data indicated that the fragmentation rate increases with ?-glucan solid concentration and interaction chamber pressure. Furthermore, since ?-glucan molecular weight is a key factor determining its biological activity, the effect of ?-glucans of different molecular weights produced by fragmentation on tumor necrosis factor (TNF)-?-stimulating activity in THP-1 human macrophage cells was investigated. Evidence suggested that ?-glucans have an immunostimulating effect on macrophage function, in the absence of cytotoxic effects. Indeed, ?-glucans characterized by a range of molecular weights produced via microfluidization exhibited promise as immunostimulatory agents. ? 2024 National Science and Technology Development Agency, THAILAND
Knowledge Taxonomy Level 1
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License
CC BY-NC
Rights
National Science and Technology Development Agency,
Publication Source
WoS