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Repetitive sequence-based PCR fingerprinting and the relationship of antimicrobial-resistance characteristics and corresponding genes among Salmonella strains from pig production
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Document Title
Repetitive sequence-based PCR fingerprinting and the relationship of antimicrobial-resistance characteristics and corresponding genes among Salmonella strains from pig production
Author
Prasertsee T., Khantaprab N., Yamsakul P., Santiyanont P., Chokesajjawatee N., Patchanee P.
Name from Authors Collection
Scopus Author ID
57189353906
Affiliations
Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand
Type
Article
Source Title
Asian Pacific Journal of Tropical Disease
ISSN
22221808
Year
2016
Volume
6
Issue
5
Page
390
Open Access
All Open Access, Bronze
Publisher
Elsevier B.V.
DOI
10.1016/S2222-1808(15)61054-4
Format
Abstract
Objective: To investigate the relationship between antimicrobial resistance characteristics and corresponding genes, and to diversify repetitive element sequence-based PCR (rep-PCR) fingerprinting of three Salmonella serotypes: Rissen, Panama and Stanley, which were isolated from pig farms and slaughterhouses in Chiang Mai and Lumphun Provinces, Thailand. Methods: A total of 90 Salmonella strains were identified using the Kauffman-White scheme. The Kirby-Bauer disk diffusion method was used to investigate resistance phenotypes of 10 antimicrobial agents. Conventional PCR was used to detect 10 antimicrobial resistance genes, additionally, rep-PCR typing method was applied to identify clonality among Salmonella isolates. Results: The antimicrobial susceptibility testing found resistance to ampicillin (80.0%), streptomycin (65.6%), tetracycline (61.1%), sulfamethoxazole (53.3%), chloramphenicol (28.9%), nalidixic acid (6.7%) and cefotaxime (2.2%). All strains were sensitive to amoxicillin-clavulanic acid, ciprofloxacin and norfloxacin. The most common antimicrobial resistance patterns among the isolates were ampicillin, chloramphenicol, streptomycin, tetracycline, and sulfamethoxazole. The type and frequency of antimicrobial genes detected included blaTEM (100.0%), aadA2 (52.2%), cmlA (45.6%), strA (38.9%), tetA(B) (16.7%), sul1 (15.6%), blaOXA-2 (14.4%), blaPSE1 (6.7%), aphA1-lab (2.2%) and blaCMY-2 (1.1%). Conclusions: Statistical analysis revealed no association between antimicrobial resistance genes and resistance profiles with the exception of cmlA and chloramphenicol, sul1 and sulfamethoxazole, aadA2 and streptomycin, and strA and streptomycin (P < 0.05). Finally, the identical groups of clonal strains were detected by rep-PCR indicating similarity among Salmonella genotypes from farms and from slaughterhouses. © 2016 Asian Pacific Tropical Medicine Press.
Keyword
Antimicrobial resistance | Genes | Rep-PCR fingerprinting | Salmonella
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency
License
N/A
Rights
N/A
Publication Source
Scopus