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A proteomic analysis of the anti-dengue virus activity of andrographolide
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Document Title
A proteomic analysis of the anti-dengue virus activity of andrographolide
Author
Paemanee A., Hitakarun A., Wintachai P., Roytrakul S., Smith D.R.
Name from Authors Collection
Affiliations
Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon Sai 4, Salaya, Nakorn Pathom 73170, Thailand; Proteomics Research Laboratory, Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand
Type
Article
Source Title
Biomedicine and Pharmacotherapy
ISSN
07533322
Year
2019
Volume
109
Page
322
Open Access
Gold
Publisher
Elsevier Masson SAS
DOI
10.1016/j.biopha.2018.10.054
Format
Abstract
Andrographolide is a major bioactive constituent of Andrographis paniculata that has been shown in vitro to have antiviral activity against a number of viruses, including the mosquito transmitted dengue virus (DENV). However, how andrographolide exerts an anti-DENV effect remains unclear. This study therefore sought to further understand the mechanism of action of andrographolide in inhibiting DENV infection of liver cells using a proteomic based approach. Both 1 dimension (D) and 2D proteome systems were used. Initial data was generated through andrographolide treatment of HepG2 cells without DENV infection (1D analysis), while subsequent data was generated through a combination of andrographolide treatment and DENV infection (2D analysis). A total of 17 (1D) and 18 (2D) proteins were identified as differentially regulated. The analyses identified proteins involved in chaperone activities, as well as energy production. In particular evidence suggested an important role for GRP78 and the unfolded protein response in mediating the anti-DENV activity of andrographolide, which might, in part, explain the broad antiviral activity of andrographolide. © 2018 Elsevier Masson SAS
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Mahidol University; Thailand Research Fund; Thailand Graduate Institute of Science and Technology
License
CC BY-NC-ND
Rights
Author
Publication Source
Scopus
Note
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