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Comparative proteomic analysis of human cholangiocarcinoma cell lines: S100A2 as a potential candidate protein inducer of invasion
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Document Title
Comparative proteomic analysis of human cholangiocarcinoma cell lines: S100A2 as a potential candidate protein inducer of invasion
Author
Wasuworawong K., Roytrakul S., Paemanee A., Jindapornprasert K., Komyod W.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok, 10400, Thailand; Proteomics Research Laboratory, Genome Institute, National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Disease Markers
ISSN
02780240
Year
2015
Volume
2015
Open Access
All Open Access, Gold, Green
Publisher
Hindawi Limited
DOI
10.1155/2015/629367
Format
Abstract
Cholangiocarcinoma (CCA) is a bile duct cancer, commonly found in Asia including Thailand and especially in the northeastern region of Thailand. To identify the proteins involved in carcinogenesis and metastasis of CCA, protein expression profiles of high-invasive KKU-M213 and low-invasive KKU-100 cell lines were compared using a comparative Ge LC-MS/MS proteomics analysis. A total of 651 differentially expressed proteins were detected of which 27 protein candidates were identified as having functions involved in cell motility. A total of 22 proteins were significantly upregulated in KKU-M213, whereas 5 proteins were downregulated in KKU-M213. S100A2, a calcium-binding protein in S100 protein family, is upregulated in KKU-M213. S100A2 is implicated in metastasis development in several cancers. The protein expression level of S100A2 was verified by Western blot analysis. Intriguingly, high-invasive KKU-M213 cells showed higher expression of S100A2 than KKU-100 cells, consistent with proteomic data, suggesting that S100A2may be a key protein involved in the progression of CCA. However, the biological function of S100A2 in cholangiocarcinoma remains to be elucidated. S100A2 might be a potential biomarker as well as a novel therapeutic target in CCA metastasis. © 2015 Kasima Wasuworawong et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
N/A
Rights
N/A
Publication Source
Scopus