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Shape and surface properties of titanate nanomaterials influence differential cellular uptake behavior and biological responses in THP-1 cells
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Document Title
Shape and surface properties of titanate nanomaterials influence differential cellular uptake behavior and biological responses in THP-1 cells
Author
Boonrungsiman S., Suchaoin W., Chetprayoon P., Viriya-empikul N., Aueviriyavit S., Maniratanachote R.
Name from Authors Collection
Affiliations
Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Khlong LuangPathum Thani 12120, Thailand; Nanomaterials for Energy and Catalysis Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Khlong LuangPathum Thani 12120, Thailand
Type
Article
Source Title
Biochemistry and Biophysics Reports
ISSN
24055808
Year
2017
Volume
9
Page
203-210
Open Access
All Open Access, Gold, Green
Publisher
Elsevier B.V.
DOI
10.1016/j.bbrep.2016.12.014
Format
Abstract
We investigated cellular uptake behavior and biological responses of spherical and fibrous titanate nanomaterials in human monocyte THP-1 cells. Two titanate nanofibers (TiNFs), namely TF-1 and TF-2, were synthesized from anatase TiO2 nanoparticles (TNPs) via hydrothermal treatment. The synthesized TiNFs and TNPs were thoroughly characterized for their size, crystallinity, surface area and surface pH. TF-1 (∼2 µm in length) was amorphous with an acidic surface, while TF-2 (∼7 µm in length) was brookite with a basic surface. The results demonstrated that none of these titanate nanomaterials resulted in significant cytotoxicity, even at the highest doses tested (50 µg/ml), consistent with an absence of ROS generation and lack of change of mitochondrial membrane potential. While no cytotoxic effect was found in the titanate nanomaterials, TF-2 tended to decrease the proliferation of THP-1 cells. Furthermore, TF-2 resulted in an inflammatory cytokine response, as evidenced by dramatic induction of IL-8 and TNF-α release in TF2 but not TF-1 nor TNPs. These results suggest that shape of titanate nanomaterials plays an important role in cellular internalization, while surface pH may play a prominent role in inflammatory response in THP-1 cells. © 2017 The Authors
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Stanford University; National Science and Technology Development Agency; National Nanotechnology Center
License
N/A
Rights
N/A
Publication Source
Scopus