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Cryptosporangium phraense sp. Nov., isolated from plant litter
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Document Title
Cryptosporangium phraense sp. Nov., isolated from plant litter
Author
Suriyachadkun C., Ngaemthao W., Pujchakarn T., Chunhametha S.
Name from Authors Collection
Affiliations
Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong LuangPathum Thani 12120, Thailand
Type
Article
Source Title
International Journal of Systematic and Evolutionary Microbiology
ISSN
14665026
Year
2020
Volume
70
Issue
2
Page
971-976
Open Access
All Open Access, Bronze
Publisher
Microbiology Society
DOI
10.1099/ijsem.0.003857
Format
Abstract
A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand. Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium. The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium. The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5% with all members of the genus Cryptosporangium. The values of DNA–DNA relatedness that distinguished this novel strain from its closest related species were below 70%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were acofriose, arabinose, galactose, glucose, mannose, xylose and ribose. The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2). The predominant fatty acids were iso-C16:0, C18:1 ω9c and anteiso-C17:0. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids. The G+C content of the genomic DNA was 71.6 mol%. Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp. nov. © 2020 The Authors.
Keyword
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Novartis Pharma; National Center for Genetic Engineering and Biotechnology
License
N/A
Rights
N/A
Publication Source
Scopus