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Towards a miniaturized culture screening for cellulolytic fungi and their agricultural lignocellulosic degradation
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Document Title
Towards a miniaturized culture screening for cellulolytic fungi and their agricultural lignocellulosic degradation
Author
Arnthong J., Siamphan C., Chuaseeharonnachai C., Boonyuen N., Suwannarangsee S.
Name from Authors Collection
Affiliations
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Klong Luang, Pathumthani, 12120, Thailand
Type
Article
Source Title
Journal of Microbiology and Biotechnology
ISSN
10177825
Year
2020
Volume
30
Issue
11
Page
1670-1679
Open Access
All Open Access, Bronze
Publisher
Korean Society for Microbiolog and Biotechnology
DOI
10.4014/JMB.2007.07005
Format
Abstract
The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products. Copyright© 2020 by The Korean Society for Microbiology and Biotechnology
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency
Funding Number
P1650036
License
N/A
Rights
N/A
Publication Source
Scopus