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Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system
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Document Title
Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system
Author
Jansaento W., Jangpatarapongsa K., Polpanich D., Wonglumsom W.
Name from Authors Collection
Affiliations
Department of Clinical Microbiology and Applied Technology, Mahidol University, Bangkok, 10700, Thailand; Center for Innovation Development and Technology Transfer, Mahidol University, Bangkok, 10700, Thailand; NANOTEC, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Food Science and Biotechnology
ISSN
12267708
Year
2016
Volume
25
Issue
1
Page
193-198
Open Access
All Open Access, Green
Publisher
Kluwer Academic Publishers
DOI
10.1007/s10068-016-0029-3
Format
Abstract
Campylobacter is an important food-borne pathogen causing acute gastroenteritis worldwide. Magnetic nanoparticle-based PCR coupled with streptavidin-horseradish peroxidase and a substrate was used for colorimetric detection. Forward primers conjugated to magnetic nanoparticles facilitated separation and concentration of Campylobacter DNA in a sample matrix. After PCR, a green color developed and was observed using the unaided eye, or detected using a spectrophotometer. High specificity and sensitivity of the 100 fg DNA/PCR reaction were achieved in pure culture experiments. The technique was applied for detection of Campylobacter on naturally contaminated chicken skin. All positive results were in agreement with results achieved using a conventional culture method. The magnetic nanoparticle-PCR-enzyme linked gene assay was practical and useful for detection of Campylobacter in complex matrices with PCR-interfering substances. © 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
Keyword
Campylobacter | Chicken | colorimetric system | magnetic nanoparticle
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
N/A
Rights
N/A
Publication Source
Scopus