-
Genetic heterogeneity of the tropical abalone (Haliotis asinina) revealed by RAPD and microsatellite analyses
- Back
Document Title
Genetic heterogeneity of the tropical abalone (Haliotis asinina) revealed by RAPD and microsatellite analyses
Author
Tang S., Popongviwat A., Klinbunga S., Tassanakajon A., Jarayabhand P., Menasveta P.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Marine Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand; Aquatic Resources Research Institute, Chulalongkorn University, Bangkok 10330, Thailand
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2005
Volume
38
Issue
2
Page
182-190
Open Access
Bronze, Green
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2005.38.2.182
Abstract
Genetic heterogeneity of the tropical abalone, Haliotis asinina was examined using randomly amplified polymorphic DNA (RAPD) and microsatellite analyses. One hundred and thirteen polymorphic RAPD fragments were generated. The percentage of polymorphic bands of H. asinina across overall primers was 85.20%. The average genetic distance of natural samples within the Gulf of Thailand (HACAME and HASAME) was 0.0219. Larger distance was observed when those samples were compared with HATRAW from the Andaman Sea (0.2309 and 0.2314). Geographic heterogeneity and FST analyses revealed population differentiation between H. asinina from the Gulf of Thailand and the Andaman Sea (p < 0.0001). Three microsatellite loci (CUHas1, CUHas4 and CUHas5) indicated relatively high genetic diversity in H. asinina (total number of alleles = 26, 5, 23 and observed heterozygosity = 0.84, 0.42 and 0.33, respectively). Significant population differentiation was also found between samples from different coastal regions (p < 0.0001). Therefore, the gene pool of natural H. asinina in coastal Thai waters can be genetically divided to 2 different populations; the Gulf of Thailand (A) and the Andaman Sea (B).
Keyword
Abalone | Genetic diversity | Microsatellites | Population differentiation | RAPD
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
Public Nuri
Rights
Korea Institute of Science and Technology Information https://www.kisti.re.kr/pageView/521?t=1648956401355
Publication Source
Scopus