-
Epizootic reptilian ferlavirus infection in individual and multiple snake colonies with additional evidence of the virus in the male genital tract
- Back
Metadata
Document Title
Epizootic reptilian ferlavirus infection in individual and multiple snake colonies with additional evidence of the virus in the male genital tract
Author
Piewbang C.,Wardhani S.W.,Poonsin P.,Yostawonkul J.,Chai-in P.,Lacharoje S.,Saengdet T.,Vasaruchapong T.,Boonrungsiman S.,Kongmakee P.,Banlunara W.,Rungsipipat A.,Kasantikul T.,Techangamsuwan S.
Name from Authors Collection
Scopus Author ID
56026006800
Affiliations
Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Animal Virome and Diagnostic Development Research Group, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; The International Graduate Course of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, 12120, Thailand; Siam Serpentarium, Siam Park Recreation Co., Ltd., Bangkok, 10520, Thailand; Snake Farm, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Bangkok, 10330, Thailand; The Zoological Park Organization under The Royal Patronage of H.M. The King, Bangkok, 10800, Thailand; Clemson Veterinary Diagnostic Center, Clemson University, Columbia, SC 29229, United States
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2021
Volume
11
Issue
1
Open Access
All Open Access, Gold, Green
Publisher
Nature Research
DOI
10.1038/s41598-021-92156-5
Abstract
Reptilian ferlavirus, a pathogen of serious concern in snakes, has been reported in Western countries, but little is known about its prevalence in Thailand, where many snake breeding farms are located. In this study, we investigated the reptilian ferlavirus via swab samples derived from 49 diseased snakes and 77 healthy snakes as well as tissue samples taken from nine dead snakes from five independent snake farms. Using molecular detection, we found the ferlavirus in 8.16% of diseased snakes, but not in healthy snakes. Out of nine farmed snakes, eight snakes derived from four farms were found to be positive. Four complete genome sequences of the ferlavirus were successfully obtained and phylogenetically clustered to the highly pathogenic ferlavirus. Tissue tropism of the ferlavirus was identified in various epithelial cell types using the in situ hybridization technique. Interestingly, the hybridization signals were strongly labeled in the male genital tract. Transmission electron microscopy was used to support the ferlaviral localization in the male genital tract. This study provides the first evidence of ferlavirus localization in the male genital tract and contributes to the knowledge about ferlavirus epidemiology, indicating that there needs to be further awareness and elucidation regarding vertical transmission of reptilian ferlavirus. © 2021, The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Publisher
Publication Source
Scopus