-
Multiploid CD61+ Cells Are the Pre-Dominant Cell Lineage Infected during Acute Dengue Virus Infection in Bone Marrow
- Back
Metadata
Document Title
Multiploid CD61+ Cells Are the Pre-Dominant Cell Lineage Infected during Acute Dengue Virus Infection in Bone Marrow
Author
Clark K.B.,Noisakran S.,Onlamoon N.,Hsiao H.-M.,Roback J.,Villinger F.,Ansari A.A.,Perng G.C.
Name from Authors Collection
Affiliations
Department of Pathology and Laboratory Medicine and the Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, United States; Medical Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok, Thailand; Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Center for Emory Bone Marrow Transplant Center, Emory University, Atlanta, GA, United States; Division of Pathology, Yerkes National Primate Research Center, Emory University, Atlanta, GA, United States; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2012
Volume
7
Issue
12
Open Access
All Open Access, Gold, Green
DOI
10.1371/journal.pone.0052902
Abstract
Depression of the peripheral blood platelet count during acute infection is a hallmark of dengue. This thrombocytopenia has been attributed, in part, to an insufficient level of platelet production by megakaryocytes that reside in the bone marrow (BM). Interestingly, it was observed that dengue patients experience BM suppression at the onset of fever. However, few studies focus on the interaction between dengue virus (DENV) and megakaryocytes and how this interaction can lead to a reduction in platelets. In the studies reported herein, BM cells from normal healthy rhesus monkeys (RM) and humans were utilized to identify the cell lineage(s) that were capable of supporting virus infection and replication. A number of techniques were employed in efforts to address this issue. These included the use of viral RNA quantification, nonstructural protein and infectivity assays, phenotypic studies utilizing immunohistochemical staining, anti-differentiation DEAB treatment, and electron microscopy. Cumulative results from these studies revealed that cells in the BM were indeed highly permissive for DENV infection, with human BM having higher levels of viral production compared to RM. DENV-like particles were predominantly observed in multi-nucleated cells that expressed CD61+. These data suggest that megakaryocytes are likely the predominant cell type infected by DENV in BM, which provides one explanation for the thrombocytopenia and the dysfunctional platelets characteristic of dengue virus infection. © 2012 Clark et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus