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Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
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Metadata
Document Title
Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
Author
Chawjiraphan W.,Apiwat C.,Segkhoonthod K.,Treerattrakoon K.,Pinpradup P.,Sathirapongsasuti N.,Pongprayoon P.,Luksirikul P.,Isarankura-Na-Ayudhya P.,Japrung D.
Name from Authors Collection
Scopus Author ID
57194972179
Affiliations
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Section of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityBangkok, Thailand; Department of Chemistry, Faculty of Science, Kasetsart UniversityBangkok, Thailand; Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart UniversityBangkok, Thailand; Department of Medical Technology, Faculty of Allied Health Science, Thammasat University, Pathumthani, Thailand; Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, United Kingdom
Type
Article
Source Title
MethodsX
ISSN
22150161
Year
2020
Volume
7
Open Access
All Open Access, Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.mex.2020.101114
Abstract
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 © 2020
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus
Note
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