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Levansucrase from bacillus amyloliquefaciens kk9 and its y237s variant producing the high bioactive levan-type fructooligosaccharides
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Metadata
Document Title
Levansucrase from bacillus amyloliquefaciens kk9 and its y237s variant producing the high bioactive levan-type fructooligosaccharides
Author
Phengnoi P.,Charoenwongpaiboon T.,Wangpaiboon K.,Klaewkla M.,Nakapong S.,Visessanguan W.,Ito K.,Pichyangkura R.,Kuttiyawong K.
Name from Authors Collection
Affiliations
Department of Chemistry, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand; Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand; Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan
Type
Article
Source Title
Biomolecules
ISSN
2218273X
Year
2020
Volume
10
Issue
5
Open Access
All Open Access, Gold, Green
Publisher
MDPI AG
DOI
10.3390/biom10050692
Abstract
Levan-typed fructooligosaccharide (LFOS), a β-2,6 linked oligofructose, displays the potential application as a prebiotic and therapeutic dietary supplement. In the present study, LFOS was synthesized using levansucrase from Bacillus amyloliquefaciens KK9 (LsKK9). The wild-type LsKK9 was cloned and expressed in E. coli, and purified by cation exchanger chromatography. Additionally, Y237S variant of LsKK9 was constructed based on sequence alignment and structural analysis to enhance the LFOS production. High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis indicated that Y237S variant efficiently produced a higher amount of short-chain LFOS than wild type. Also, the concentration of enzyme and sucrose in the reactions was optimized. Finally, prebiotic activity assay demonstrated that LFOS produced by Y237S variant had higher prebiotic activity than that of the wild-type enzyme, making the variant enzyme attractive for food biotechnology. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus