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High performance dengue virus antigen-based serotyping-ns1-elisa (Plus): A simple alternative approach to identify dengue virus serotypes in acute dengue specimens
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Metadata
Document Title
High performance dengue virus antigen-based serotyping-ns1-elisa (Plus): A simple alternative approach to identify dengue virus serotypes in acute dengue specimens
Author
Prommool T.,Sethanant P.,Phaenthaisong N.,Tangthawornchaikul N.,Songjaeng A.,Avirutnan P.,Mairiang D.,Luangaram P.,Srisawat C.,Kasinrerk W.,Vasanawathana S.,Sriruksa K.,Limpitikul W.,Malasit P.,Puttikhunt C.
Name from Authors Collection
Scopus Author ID
6507262736
Scopus Author ID
57218341452
Affiliations
Molecular Biology of Dengue and Flaviviruses Research Team, Medical Molecular Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok, Thailand; Division of Dengue Hemorrhagic Fever Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center of Research Excellence in Dengue and Emerging Pathogens, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency, Chiang Mai, Thailand; Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand; Khon Kaen Hospital, Khon Kaen, Thailand; Songkhla Hospital, Songkhla, Thailand
Type
Article
Source Title
PLoS Neglected Tropical Diseases
ISSN
19352727
Year
2021
Volume
15
Issue
2
Open Access
All Open Access, Gold, Green
Publisher
Public Library of Science
DOI
10.1371/journal.pntd.0009065
Abstract
Dengue hemorrhagic fever (DHF) is caused by infection with dengue virus (DENV). Four different serotypes (DENV1-4) co-circulate in dengue endemic areas. The viral RNA genome-based reverse-transcription PCR (RT-PCR) is the most widely used method to identify DENV serotypes in patient specimens. However, the non-structural protein 1 (NS1) antigen as a biomarker for DENV serotyping is an emerging alternative method. We modified the serotyping-NS1-enzyme linked immunosorbent assay (stNS1-ELISA) from the originally established assay which had limited sensitivity overall and poor specificity for the DENV2 serotype. Here, four biotinylated serotype-specific antibodies were applied, including an entirely new design for detection of DENV2. Prediction of the infecting serotype of retrospective acute-phase plasma from dengue patients revealed 100% concordance with the standard RT-PCR method for all four serotypes and 78% overall sensitivity (156/200). The sensitivity of DENV1 NS1 detection was greatly improved (from 62% to 90%) by the addition of a DENV1/DENV3 sub-complex antibody pair. Inclusive of five antibody pairs, the stNS1-ELISA (plus) method showed an overall increased sensitivity to 85.5% (171/200). With the same clinical specimens, a commercial NS1 rapid diagnostic test (NS1-RDT) showed 72% sensitivity (147/200), significantly lower than the stNS1-ELISA (plus) performance. In con-clusion, the stNS1-ELISA (plus) is an improved method for prediction of DENV serotype and for overall sensitivity. It could be an alternative assay not only for early dengue diagnosis, but also for serotype identification especially in remote resource-limited dengue endemic areas. © 2021 Prommool et al.
Industrial Classification
Knowledge Taxonomy Level 1
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Knowledge Taxonomy Level 3
License
CC BY
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Publication Source
Scopus