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Study of the lipolysis effect of nanoliposome-encapsulated Ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach
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Metadata
Document Title
Study of the lipolysis effect of nanoliposome-encapsulated Ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach
Author
Krobthong S.,Yingchutrakul Y.,Visessanguan W.,Mahatnirunkul T.,Samutrtai P.,Chaichana C.,Papan P.,Choowongkomon K.
Name from Authors Collection
Affiliations
Interdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok, 10900, Thailand; National Omics Center, NSTDA, Pathum Thani, 12120, Thailand; National Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani, 12120, Thailand; National Nanotechnology Center, NSTDA, Pathum Thani, 12120, Thailand; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand; Siriraj Center of Research Excellence for Diabetes and Obesity (SiCORE-DO), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand; Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok, 10900, Thailand
Type
Article
Source Title
Foods
ISSN
23048158
Year
2021
Volume
10
Issue
9
Open Access
All Open Access, Gold, Green
Publisher
MDPI
DOI
10.3390/foods10092157
Abstract
Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X1) and enzyme usage (X2) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X1 at 8.63 h and X2 at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nanoliposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity. © 2021 by the authors.
Keyword
3T3-L1 | Hypolipidemic activity | Lingzhi | Peptides | RSM
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus