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Immersion vaccination by a biomimetic-mucoadhesive nanovaccine induces humoral immune response of red tilapia (Oreochromis sp.) against flavobacterium columnare challenge
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Metadata
Document Title
Immersion vaccination by a biomimetic-mucoadhesive nanovaccine induces humoral immune response of red tilapia (Oreochromis sp.) against flavobacterium columnare challenge
Author
Kitiyodom S.,Yata T.,Thompson K.D.,Costa J.,Elumalai P.,Katagiri T.,Temisak S.,Namdee K.,Rodkhum C.,Pirarat N.
Name from Authors Collection
Affiliations
Wildlife Exotic Aquatic Animal Pathology-Research Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, United Kingdom; School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies, Kochi, Kerala, 682506, India; Laboratory of Fish Health Management, Course of Aquatic Biosciences, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan; Bio Analysis Group, Chemical Metrology and Biometry Department, National Institute of Metrology (NIMT), Pathum Thani12120, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani12120, Thailand; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Vaccines
ISSN
2076393X
Year
2021
Volume
9
Issue
11
Open Access
All Open Access, Gold, Green
Publisher
MDPI
DOI
10.3390/vaccines9111253
Abstract
Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against columnaris disease, a serious bacterial disease in farmed red tilapia caused by Flavobacterium columnare. However, the induction of a systemic immune response by the vaccine is yet to be investigated. Here, we examine if a specific hu-moral immune response is stimulated in tilapia by a biomimetic-mucoadhesive nanovaccine against Flavobacterium columnare using an indirect-enzyme-linked immunosorbent assay (ELISA), serum bactericidal activity (SBA) and the expression of immune-related genes within the head-kidney and spleen, together with assessing the relative percent survival of vaccinated fish after experimentally infecting them with F. columnare. The anti-IgM antibody titer of fish at 14 and 21 days post-vaccination was significantly higher in chitosan complex nanoemulsion (CS-NE) vaccinated fish compared to fish vaccinated with the formalin-killed vaccine or control fish, supporting the serum bactericidal activity results at these time points. The cumulative mortality of the unvaccinated control fish was 87% after challenging fish with the pathogen, while the cumulative mortality of the CS-NE vaccinated group was 24%, which was significantly lower than the formalin-killed vaccinated and control fish. There was a significant upregulation of IgM, IgT, TNF α, and IL1-β genes in the spleen and kidney of vaccinated fish. Significant upregulation of IgM and IgT genes was observed in the spleen of CS-NE vaccinated fish. The study confirmed the charged-chitosan-based mucoadhesive nanovaccine to be an effective platform for immersion vaccination of tilapia, with fish generating a humoral systemic immune response against columnaris disease in vaccinated fish. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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License
CC BY
Rights
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Publication Source
Scopus