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Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito
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Metadata
Document Title
Identification of New Protein Interactions between Dengue Fever Virus and Its Hosts, Human and Mosquito
Author
Mairiang D.,Zhang H.,Sodja A.,Murali T.,Suriyaphol P.,Malasit P.,Limjindaporn T.,Finley Jr. R.L.
Name from Authors Collection
Affiliations
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, United States; Department of Biology, Wayne State University, Detroit, MI, United States; Bioinformatics and Data Management for Research Unit, Faculty of Medicine Siriraj Hospital, and Center for Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Thailand; Dengue Hemorrhagic Fever Research Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Medical Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok, Thailand; Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI, United States; Medical Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok, Thailand
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2013
Volume
8
Issue
1
Open Access
All Open Access, Gold, Green
DOI
10.1371/journal.pone.0053535
Abstract
The four divergent serotypes of dengue virus are the causative agents of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of the world's population live in areas where dengue is prevalent, and thousands of deaths are caused by the viruses every year. Dengue virus is transmitted from one person to another primarily by the yellow fever mosquito, Aedes aegypti. Recent studies have begun to define how the dengue viral proteins interact with host proteins to mediate viral replication and pathogenesis. A combined analysis of these studies, however, suggests that many virus-host protein interactions remain to be identified, especially for the mosquito host. In this study, we used high-throughput yeast two-hybrid screening to identify mosquito and human proteins that physically interact with dengue proteins. We tested each identified host protein against the proteins from all four serotypes of dengue to identify interactions that are conserved across serotypes. We further confirmed many of the interactions using co-affinity purification assays. As in other large-scale screens, we identified some previously detected interactions and many new ones, moving us closer to a complete host - dengue protein interactome. To help summarize and prioritize the data for further study, we combined our interactions with other published data and identified a subset of the host-dengue interactions that are now supported by multiple forms of evidence. These data should be useful for understanding the interplay between dengue and its hosts and may provide candidates for drug targets and vector control strategies. © 2013 Mairiang et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Human Genome Research Institute
License
CC BY
Rights
Author
Publication Source
Scopus