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Construction and application of a protein interaction map for white spot syndrome virus (WSSV)
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Metadata
Document Title
Construction and application of a protein interaction map for white spot syndrome virus (WSSV)
Author
Sangsuriya P.,Huang J.-Y.,Chu Y.-F.,Phiwsaiya K.,Leekitcharoenphon P.,Meemetta W.,Senapin S.,Huang W.-P.,Withyachumnarnkul B.,Flegel T.W.,Lo C.-F.
Name from Authors Collection
Affiliations
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand; Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand; Institute of Zoology, National Taiwan University, Taipei, Taiwan; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Shrimp Genetic Improvement Center, Surat Thani 84100, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Institute of Bioinformatics and Biosignal Transduction, College of Bioscience and Biotechnology, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan
Type
Article
Source Title
Molecular and Cellular Proteomics
ISSN
15359476
Year
2014
Volume
13
Issue
1
Open Access
All Open Access, Hybrid Gold, Green
DOI
10.1074/mcp.M113.029199
Abstract
White spot syndrome virus (WSSV) is currently the most serious global threat for cultured shrimp production. Although its large, double-stranded DNA genome has been completely characterized, most putative protein functions remain obscure. To provide more informative knowledge about this virus, a proteomic-scale network of WSSVWSSV protein interactions was carried out using a comprehensive yeast two-hybrid analysis. An array of yeast transformants containing each WSSV open reading frame fused with GAL4 DNA binding domain and GAL4 activation domain was constructed yielding 187 bait and 182 prey constructs, respectively. On screening of ∼28,000 pairwise combinations, 710 interactions were obtained from 143 baits. An independent coimmunoprecipitation assay (co-IP) was performed to validate the selected protein interaction pairs identified from the yeast two-hybrid approach. The program Cytoscape was employed to create a WSSV protein-protein interaction (PPI) network. The topology of the WSSV PPI network was based on the Barabási-Albert model and consisted of a scale-free network that resembled other established viral protein interaction networks. Using the RNA interference approach, knocking down either of two candidate hub proteins gave shrimp more protection against WSSV than knocking down a nonhub gene. The WSSV protein interaction map established in this study provides novel guidance for further studies on shrimp viral pathogenesis, host-viral protein interaction and potential targets for therapeutic and preventative antiviral strategies in shrimp aquaculture. Molecular & Cellular Proteomics 13: 10.1074/mcp.M113.029199, 269-282, 2014. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus