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Side-by-side comparison of commonly used biomolecules that differ in size and affinity on tumor uptake and internalization
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Metadata
Document Title
Side-by-side comparison of commonly used biomolecules that differ in size and affinity on tumor uptake and internalization
Author
Leelawattanachai J.,Kwon K.-W.,Michael P.,Ting R.,Kim J.-Y.,Jin M.M.
Name from Authors Collection
Affiliations
Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, United States; Department of Radiology, Weill Cornell Medical College, New York, NY 10065, United States; Department of Advanced Materials Engineering, Kangwon National University, Samcheok, South Korea; National Nanotechnology Center, National Science and Technology Development Agency, Pathumthani, 12120, Thailand; Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South Korea
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2015
Volume
10
Issue
4
Open Access
All Open Access, Gold, Green
Publisher
Public Library of Science
DOI
10.1371/journal.pone.0124440
Abstract
The ability to use a systemically injected agent to image tumor is influenced by tumor characteristics such as permeability and vascularity, and the size, shape, and affinity of the imaging agent. In this study, six different imaging biomolecules, with or without specificity to tumor, were examined for tumor uptake and internalization at the whole body, ex-vivo tissue, and cellular levels: antibodies, antibody fragments (Fab), serum albumin, and streptavidin. The time of peak tumor uptake was dependent solely on the size of molecules, suggesting that molecular size is the major factor that influences tumor uptake by its effect on systemic clearance and diffusion into tumor. Affinity to tumor antigen failed to augment tumor uptake of Fab above non-specific accumulation, which suggests that Fab fragments of typical monoclonal antibodies may fall below an affinity threshold for use as molecular imaging agents. Despite abundant localization into the tumor, albumin and streptavidin were not found on cell surface or inside cells. By comparing biomolecules differing in size and affinity, our study highlights that while pharmacokinetics are a dominant factor in tumor uptake for biomolecules, affinity to tumor antigen is required for tumor binding and internalization. © 2015 Leelawattanachai et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Institutes of Health; National Cancer Institute; Ministry of Science, ICT and Future Planning
License
CC BY
Rights
Author
Publication Source
Scopus