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Detection and validation of EST-SSR markers associated with sugar-related traits in sugarcane using linkage and association mapping
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Metadata
Document Title
Detection and validation of EST-SSR markers associated with sugar-related traits in sugarcane using linkage and association mapping
Author
Ukoskit K.,Posudsavang G.,Pongsiripat N.,Chatwachirawong P.,Klomsa-ard P.,Poomipant P.,Tragoonrung S.
Name from Authors Collection
Affiliations
Department of Biotechnology, Thammasat University, (Rangsit Campus) Klong Luang, Pathum Thani, 12121, Thailand; Department of Agronomy, Faculty of Agriculture, Kasetsart University, (Kamphaengsean Campus), Nakhon Pathom73140, Thailand; Mitr Phol Innovation and Research Centre, 399 Moo 1, Chumphae-Phukiao Rd. Khoksa-at, Phu Khiao, Chaiyaphum, 36110, Thailand; Institute of Food Research and Product Development, Kasetsart University, P.O. Box 1043, Kasetsart, Chatuchak, Bangkok, 10903, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Khlong Luang, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Genomics
ISSN
08887543
Year
2019
Volume
111
Issue
1
Open Access
All Open Access, Bronze
Publisher
Academic Press Inc.
DOI
10.1016/j.ygeno.2018.03.019
Abstract
Sugar-related traits are of great importance in sugarcane breeding. In the present study, quantitative trait loci (QTL) mapping validated with association mapping was used to identify expressed sequence tag-simple sequence repeats (EST-SSRs) associated with sugar-related traits. For linkage mapping, 524 EST-SSRs, 241 Amplified Fragment Length Polymorphisms, and 10 genomic SSR markers were mapped using 283 F 1 progenies derived from an interspecific cross. Six regions were identified using Multiple QTL Mapping, and 14 unlinked markers using single marker analysis. Association analysis was performed on a set of 200 accessions, based on the mixed linear model. Validation of the EST-SSR markers using association mapping within the target QTL genomic regions identified two EST-SSR markers showing a putative relationship with uridine diphosphate (UDP) glycosyltransferase, and beta-amylase, which are associated with pol and sugar yield. These functional markers can be used for marker-assisted selection of sugarcane. © 2018 Elsevier Inc.
Keyword
Association mapping | QTL mapping | Sugar-related traits | Sugarcane
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
University of Illinois at Urbana-Champaign; International Centre for Genetic Engineering and Biotechnology; National Center for Genetic Engineering and Biotechnology
License
CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus