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Comparative proteomic analysis of mature and immature oocytes in domestic cats
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Document Title
Comparative proteomic analysis of mature and immature oocytes in domestic cats
Author
Turathum B.,Saikhun K.,Roytrakul S.,Changsangfa C.,Tanasawet S.,Sroyraya M.,Kitiyanant Y.
Name from Authors Collection
Affiliations
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand; Department of Basic Medical Science, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok, 10300, Thailand; Institute of Molecular Biosciences, Mahidol University, NakhonPathom, 73170, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand; Department of Anatomy, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand
Type
Article
Source Title
Asian Pacific Journal of Reproduction
ISSN
23050500
Year
2020
Volume
9
Issue
1
Open Access
All Open Access, Gold
Publisher
Wolters Kluwer Medknow Publications
DOI
10.4103/2305-0500.275525
Abstract
Objective: To evaluate changes of feline (Felis catus) oocytes proteins during in vitro maturation by using the proteomic approach. Methods: Immature oocytes (germinal vesicle) isolated from female cats were cultured and collected at 0 h and 24 h. After collection, oocytes were investigated into immature (germinal vesicle) and mature (metaphase II) stages. The qualitative profiles of the proteins at the immature and mature stages were determined by one-dimensional electrophoresis and liquid chromatography-mass spectrometry. Results: Our data revealed that following 24 h in vitro maturation the maturation rate (metaphase II stage) was 58.7%. Eighty-one of the 260 proteins analyzed were differentially expressed between the germinal vesicle stage and the metaphase II-Arrest stage. Proteomic analysis of germinal vesicle and metaphase II oocytes showed abundant expression of proteins involved in transportation (10%), indicating that this was a major characteristic of germinal vesicle oocytes. Similarly, analysis of the proteome of metaphase II oocytes indicated that cell cycle proteins were overexpressed. Interestingly, proteins involved in DNA repair and apoptosis were only expressed in germinal vesicle oocytes and proteins involved in fertilization were only expressed in metaphase II oocytes. Conclusions: The overexpression of certain proteins in germinal vesicle and metaphase II is necessary for oocyte development and maturation. Our findings provide a valuable resource for further investigations into protein expression in oocytes at different developmental stages. ©2020 Asian Pacific Journal of Reproduction Produced by Wolters Kluwer-Medknow. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-SA
Rights
Wolters Kluwer Medknow Publications
Publication Source
Scopus
Note
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