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Optimization of production parameters for andrographolide-loaded nanoemulsion preparation by microfluidization and evaluations of its bioactivities in skin cancer cells and uvb radiation-exposed skin
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Document Title
Optimization of production parameters for andrographolide-loaded nanoemulsion preparation by microfluidization and evaluations of its bioactivities in skin cancer cells and uvb radiation-exposed skin
Author
Asasutjarit R.,Sooksai N.,Fristiohady A.,Lairungruang K.,Ng S.-F.,Fuongfuchat A.
Name from Authors Collection
Affiliations
Thammasat University Research Unit in Drug, Health Product Development and Application (DHP-DA), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat University, Pathum Thani12120, Thailand; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari, 93132, Indonesia; Department of Applied Thai Traditional Medicine, Praboromarajchanok Institute, Abhaibhubejhr College of Thai Traditional Medicine Prachinburi, Prachinburi25230, Thailand; Centre for Drug Delivery Technology, Faculty of Pharmacy, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, 50300, Malaysia; National Metal and Materials Technology Center, National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani12120, Thailand
Type
Article
Source Title
Pharmaceutics
ISSN
19994923
Year
2021
Volume
13
Issue
8
Open Access
All Open Access, Gold, Green
Publisher
MDPI AG
DOI
10.3390/pharmaceutics13081290
Abstract
Andrographolide (AG) is an active compound isolated from Andrographis paniculata (Family Acanthaceae). Although it possesses beneficial bioactivities to the skin, there is insufficient information of its applications for treatment of skin disorders due to low water solubility leading to complications in product development. To overcome the problem, an AG-loaded nanoemulsion (AG-NE) was formulated and prepared using a microfluidization technique. This study aimed to investigate the effect of pressure and the number of homogenization cycles (factors) on droplet size, polydispersity index and zeta potential of AG-NE (responses) and to determine the effect of AG-NE on skin cancer cells and UVB irradiation-induced skin disorders in rats. Relationships between factors versus responses obtained from the face-centered central composite design were described by quadratic models. The optimum value of parameters for the production of optimized AG-NE (Op-AG-NE) were 20,000 psi of pressure and 5 homogenization cycles. Op-AG-NE showed promising cytotoxicity effects on the human malignant melanoma-(A375 cells) and non-melanoma cells (A-431 cells) via apoptosis induction with a high selectivity index and also inhibited intracellular tyrosinase activity in the A375 cells. Op-AG-NE could reduce melanin index and healed UVB irradiation exposed skin. Op-AG-NE thus had potential for treatment of skin cancers and skin disorders from exposure to UVB radiation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Thammasat University
License
CC BY
Rights
Author
Publication Source
Scopus