-
Its2 sequencing and targeted meta-proteomics of infant gut mycobiome reveal the functional role of rhodotorula sp. During atopic dermatitis manifestation
- Back
Document Title
Its2 sequencing and targeted meta-proteomics of infant gut mycobiome reveal the functional role of rhodotorula sp. During atopic dermatitis manifestation
Author
Mok K.,Suratanon N.,Roytrakul S.,Charoenlappanit S.,Patumcharoenpol P.,Chatchatee P.,Vongsangnak W.,Nakphaichit M.
Name from Authors Collection
Affiliations
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand; Specialized Research Unit: Probiotics and Prebiotics for Health, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand; Pediatric Allergy & Clinical Immunology Research Unit, Division of Allergy and Immunology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok, 10330, Thailand; Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani12120, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, 10900, Thailand
Type
Article
Source Title
Journal of Fungi
ISSN
2309608X
Year
2021
Volume
7
Issue
9
Open Access
All Open Access, Gold, Green
Publisher
MDPI
DOI
10.3390/jof7090748
Abstract
Association between the gut mycobiome and atopic dermatitis was investigated in 9–12-month-old infants using metagenomics. Two groups of atopic dermatitis infants were classified according to their symptom development as outgrown (recovered) and persisted (still undergoing). The evenness and diversity of the mycobiome in the persisted group were higher than in the healthy and outgrown groups. Dysbiosis of the microbiome in the persisted group was observed by a reduction in the Ascomycota/Basidiomycota ratio. Five fungi were selected as markers from each sample group. In the persisted group, Rhodotorula sp. abundance increased significantly, while Wickerhamomyces sp. and Kodamaea sp. abundance increased in the healthy group, and Acremonium sp. and Rhizopus sp. abundance increased considerably in the outgrown group. Metaproteomic analysis revealed that the persisted group had a high abundance of fungal proteins, particularly those from Rhodotorula sp. Unique proteins such as RAN-binding protein 1 and glycerol kinase from Rhodotorula sp. were hypothesized to be related to atopic dermatitis manifestation in infants. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
Atopic dermatitis | Fungi | Gut–skin axis | Meta-proteomics | Mycobiome | Rhodotorula
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Department of Biotechnology, Ministry of Science and Technology, India; National Science and Technology Development Agency; Kasetsart University; Kasetsart University Research and Development Institute; National Center for Genetic Engineering and Biotechnology
License
CC BY
Rights
Author
Publication Source
Scopus