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A group of infection-enhancing and focus size-reducing monoclonal antibodies recognized an 慳 and c� strands epitope in the pr domain of Dengue Virus prM
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Document Title
A group of infection-enhancing and focus size-reducing monoclonal antibodies recognized an 慳 and c� strands epitope in the pr domain of Dengue Virus prM
Author
Keelapang P. Supasa P. Sriburi R. Puttikhunt C. Cardosa J. Kasinrerk W. Malasit P. Sittisombut N.
Affiliations
Department of Microbiology Faculty of Medicine Chiang Mai University Chiang Mai Thailand; Medical Biotechnology Research Unit. National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Bangkok Thailand; Molecular Biology of Dengue and Flaviviruses Research Team Medical Molecular Biotechnology Research Group National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Pathumthani Thailand; Division of Dengue Hemorrhagic Fever Research and Siriraj Center of Research Excellence in Dengue and Emerging Pathogens Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand; Institute of Health and Community Medicine Universiti Malaysia Sarawak Sarawak Kuching Malaysia; Division of Clinical Immunology Department of Medical Technology Faculty of Associated Medical Sciences Chiang Mai University Chiang Mai Thailand; Biomedical Technology Research Center National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency at the Faculty of Associated Medical Sciences Chiang Mai University Chiang Mai Thailand
Type
Article
Source Title
Virus Research
ISSN
1681702
Year
2023
Volume
323
Open Access
All Open Access Hybrid Gold Green
Publisher
Elsevier B.V.
DOI
10.1016/j.virusres.2022.199015
Abstract
Partial cleavage of a dengue virus envelope protein prM by furin results in a mixture of extracellular particles with variable levels of maturation and infectivity. Partially mature particles can infect leukocytes via interaction between the prM-anti-prM antibody complex with Fc? receptors. Known prM epitopes involved in antibody-mediated infection are localized to the pr domain. In this study a group of murine anti-prM monoclonal antibodies with strong infection-enhancing activity was found to reduce the focus size of subsets of multiple dengue serotypes that they could enhance. By employing sets of overlapping peptides four antibodies recognizing 2-mercaptoethanol-insensitive epitopes were mapped to a common tetrapeptide located distantly in the b-c loop and furin binding site. Substitution mutations of each or both of the tetrapeptides in virus-like particles however failed to reduce binding. Further mapping experiments were performed using immature virus-like particles with abolished furin binding site to minimize the differential influence of various pr substitutions on pr-M cleavage. Reduction of antibody binding was detected when single alanine substitutions were introduced into the 慳� strand and 慶 strand of pr domain. These findings suggest that the pr 慳 and c strands region is the major binding site of these unusual focus size-reducing anti-prM antibodies. ? 2022 The Authors
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-ND
Rights
Authors
Publication Source
WOS