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A Mucor rouxii mutant with high accumulation of an unusual trans-linoleic acid (9c,12t-C18 : 2)
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Document Title
A Mucor rouxii mutant with high accumulation of an unusual trans-linoleic acid (9c,12t-C18 : 2)
Author
Laoteng K, Pongchuachidthai R, Rueksomtawin K, Dandusitapunth Y, Tanticharoen M, Cheevadhanarak S
Name from Authors Collection
Affiliations
King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); King Mongkuts University of Technology Thonburi
Type
Article
Source Title
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097
Year
2003
Volume
223
Open Access
Bronze
Publisher
OXFORD UNIV PRESS
DOI
10.1016/S0378-1097(03)00324-0
Format
Abstract
Genetic and biochemical approaches reveal the existence of a gamma-linolenic acid biosynthetic pathway in Mucor rouxii. By treatment with ultraviolet light, combined with low temperature cultivation and filtration enrichment, a mutant defective in polyunsaturated fatty acid synthesis was isolated. Genetic analysis and fatty acid supplementation indicate that the defect occurred in the Delta(12)-desaturation resulting in the absence of cis-linoleic acid and gamma-linolenic acid and in the accumulation of monounsaturated fatty acids. In addition, an unusual fatty acid, trans-linoleic acid (9c,12t-C18:2), which has not been reported previously in this fungus, was found to increase in the mutant. The information gained from the mutant was used to develop the hypothetical pathway of fatty acid desaturation in M. rouxii. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Rights
Publisher
Publication Source
WOS