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A novel portable Raman scattering platform for antibiotic screening in pig urine
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Document Title
A novel portable Raman scattering platform for antibiotic screening in pig urine
Author
Wattanavichean N. Nimittrakoolchai O.-U. Nuntawong N. Horprathum M. Eiamchai P. Limwichean S. Somboonsaksri P. Sreta D. Meesuwan S.
Affiliations
School of Materials Science and Innovation Faculty of Science Mahidol University Nakhon Pathom Phutthamonthon Thailand; SCI Innovatech Co. Ltd Amphur Mueang Bangkhasor Nonthaburi Thailand; National Electronics and Computer Technology Center National Science and Technology Development Agency Pathum Thani Khlong Luang Thailand; Faculty of Veterinary Medicine Rajamangala University of Technology Tawan-ok Chonburi Sriracha Thailand
Type
Article
Source Title
Veterinary World
ISSN
9728988
Year
2023
Volume
16
Issue
1
Page
204-214
Open Access
All Open Access Gold Green
Publisher
Veterinary World
DOI
10.14202/vetworld.2023.204-214
Abstract
Background and Aim: Public health and food safety are gaining attention globally. Consumer health can be protected from chemical residues in meat by early detection or screening for antibiotic residues before selling the meat commercially. However conventional practices are normally applied after slaughtering which leads to massive business losses. This study aimed to use portable surface-enhanced Raman spectroscopy (SERS) equipped with multivariate curve resolution-alternation least squares (MCR-ALS) to determine the concentrations of enrofloxacin oxytetracycline and neomycin concentrations. This approach can overcome the problems of business loss costs and time-consumption and limit of detection (LOD). Materials and Methods: Aqueous solutions of three standard antibiotics (enrofloxacin oxytetracycline and neomycin) with different concentrations were prepared and the LOD for each antibiotic solution was determined using SERS. Extracted pig urine was spiked with enrofloxacin at concentrations of 10 20 50 100 and 10 000 ppm. These solutions were investigated using SERS and MCR-ALS analysis. Urine samples from pigs at 1 and 7 days after enrofloxacin administration were collected and investigated using SERS and MCR-ALS to differentiate the urinary enrofloxacin concentrations. Results: The LOD of enrofloxacin oxytetracycline and neomycin in aqueous solutions were 0.5 2.0 and 100 ppm respectively. Analysis of enrofloxacin spiking in pig urine samples demonstrated the different concentrations of enrofloxacin at 10 20 50 100 and 10 000 ppm. The LOD of spiking enrofloxacin was 10 ppm which was 10 times lower than the regulated value. This technique was validated for the first time using urine collected on days 1 and 7 after enrofloxacin administration. The results revealed a higher concentration of enrofloxacin on day 7 than on day 1 due to consecutive administrations. The observed concentration of enrofloxacin was closely correlated with its circulation time and metabolism in pigs. Conclusion: A combination of SERS sensing platform and MCR-ALS is a promising technique for on-farming screening. This platform can increase the efficiency of antibiotic detection in pig urine at lower costs and time. Expansion and fine adjustments of the Raman dataset may be required for individual farms to achieve higher sensitivity. Copyright: Wattanavichean et al.
Keyword
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS