A wild rice-derived peptide R14 ameliorates monosodium urate crystals-induced IL-1? secretion through inhibition of NF-?B signaling and NLRP3 inflammasome activation

Abstract

Gout is an inflammatory arthritis initiated by the deposition of monosodium urate crystals (MSU) around the joints and surrounding tissues. MSU crystals activate the nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome to the release of interleukin-1? (IL-1?). Gout can have a substantial impact on patient s quality of life and currently available medicines are unable to meet all the clinical needs. This study explored anti-gout potentials of the Rice14 (R14) peptide a peptide derived from leaves of wild rice Oryza minuta. The effects of R14 peptide on IL-1? secretion in THP-1 macrophages with MSU crystals-induced inflammation were examined. Our results clearly showed that the R14 peptide significantly inhibited the secretion of IL-1? in MSU crystals-induced macrophages and the effects were dose-related. For safety testing the R14 peptide did not show both cytotoxicity and hemolytic activity. In addition the R14 peptide strongly suppressed the phospho-I?B-? and nuclear factor kappa-B (NF-?B) p65 proteins in NF-?B signaling pathway reduced the NLRP3 expression and inhibited the MSU crystals-mediated cleavage of caspase-1 as well as mature IL-1?. The R14 peptide also reduced MSU-triggered intracellular ROS levels in macrophages. Taken together these results indicated that R14 peptide inhibited MSU crystals-induced IL-1? production through NF-?B and NLRP3 inflammasome activation. Our findings demonstrated that R14 peptide the newly recognized peptide from wild rice possessed potent regulatory activity against IL-1? production in MSU crystals-induced inflammation and we therefore propose that the R14 peptide is a promising molecule with potential clinical application in the treatment of MSU crystals-induced inflammation. Copyright ? 2023 Charoenwutthikun et al.