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Abalone collagen extracts potentiate stem cell properties of human epidermal keratinocytes
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Document Title
Abalone collagen extracts potentiate stem cell properties of human epidermal keratinocytes
Author
Thaweekitphathanaphakdee S., Chanvorachote P., Prateepchinda S., Khongkow M., Sucontphunt A.
Name from Authors Collection
Scopus Author ID
57197722491
Affiliations
College of Pharmacy, Rangsit University, Pathum Thani, 12000, Thailand; Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; Nation Nanotechnology Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Herbal Medicinal Products Research and Development Center, College of Pharmacy, Rangsit University, Pathum Thani, 12000, Thailand
Type
Article
Source Title
Marine Drugs
ISSN
16603397
Year
2019
Volume
17
Issue
7
Open Access
All Open Access, Gold, Green
Publisher
MDPI AG
DOI
10.3390/md17070424
Format
Abstract
Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products. © 2019 by the authors.
Keyword
Abalone collagen extract | Keratinocytes | MTT | Stemness | Wound healing
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; Rangsit University
License
CC BY
Rights
Author
Publication Source
Scopus