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ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging
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Document Title
ABO Blood-Typing Using an Antibody Array Technique Based on Surface Plasmon Resonance Imaging
Author
Houngkamhang N, Vongsakulyanon A, Peungthum P, Sudprasert K, Kitpoka P, Kunakorn M, Sutapun B, Amarit R, Somboonkaew A, Srikhirin T
Name from Authors Collection
Affiliations
Mahidol University; Mahidol University; Suranaree University of Technology; Suranaree University of Technology; National Science & Technology Development Agency - Thailand; National Electronics & Computer Technology Center (NECTEC); Mahidol University
Type
Article
Source Title
SENSORS
ISSN
1424-8220
Year
2013
Volume
13
Issue
9
Page
11913-11922
Open Access
Green Published, Green Submitted, gold
Publisher
MDPI AG
DOI
10.3390/s130911913
Format
Abstract
In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%-68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
National Nanotechnology Center (NANOTEC), NSTDA, Ministry of Science and Technology, Thailand through its program of Center of Excellence Network; Thailand Research Fund (TRF) through the Royal Golden Jubilee Ph.D. Program [PHD/0242/2549]; Thai Red Cross Society
License
CC BY
Rights
Authors
Publication Source
WOS