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Actin interacts with dengue virus 2 and 4 envelope proteins
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Document Title
Actin interacts with dengue virus 2 and 4 envelope proteins
Author
Jitoboam K., Phaonakrop N., Libsittikul S., Thepparit C., Roytrakul S., Smith D.R.
Name from Authors Collection
Affiliations
Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, Thailand; Center for Emerging and Neglected Infectious Diseases, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2016
Volume
11
Issue
3
Open Access
All Open Access, Gold, Green
Publisher
Public Library of Science
DOI
10.1371/journal.pone.0151951
Format
Abstract
Dengue virus (DENV) remains a significant public health problem in many tropical and subtropical countries worldwide. The DENV envelope (E) protein is the major antigenic determinant and the protein that mediates receptor binding and endosomal fusion. In contrast to some other DENV proteins, relatively few cellular interacting proteins have been identified. To address this issue a co-immuoprecipitation strategy was employed. The predominant co-immunoprecipitating proteins identified were actin and actin related proteins, however the results suggested that actin was the only bona fide interacting partner. Actin was shown to interact with the E protein of DENV 2 and 4, and the interaction between actin and DENV E protein was shown to occur in a truncated DENV consisting of only domains I and II. Actin was shown to decrease during infection, but this was not associated with a decrease in gene transcription. Actin-related proteins also showed a decrease in expression during infection that was not transcriptionally regulated. Cytoskeletal reorganization was not observed during infection, suggesting that the interaction between actin and E protein has a cell type specific component. © 2016 Jitoboam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Office of the Higher Education Commission; Mahidol University; Thailand Research Fund; Thailand Graduate Institute of Science and Technology
License
N/A
Rights
N/A
Publication Source
Scopus