Abstract
A novel filamentous bacterial strain, A-T 5190T, which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5190T belonged to the genus Actinoplanes and was related most closely to Actinoplanes palleronii NBRC 14916T (98.88% similarity) and Actinoplanes rectilineatus NBRC 13941T (98.54%). DNA–DNA relatedness values between strain A-T 5190T and its closest relatives were below 70%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The wholecell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acid C17:1 and branched fatty acids iso-C16:0, iso-C15:0 and anteiso-C17:0. The G+C content of the genomic DNA was 71.9 mol%. Evidence from phenotypic, chemotaxonomic and genotypic studies indicate that strain A-T 5190T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes luteus sp. nov. is proposed. The type strain is A-T 5190T (=BCC 41582T=NBRC 109644T). © 2015 IUMS.
