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Alterations of mineralized matrix by lead exposure in osteoblast (MC3T3-E1) culture
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Document Title
Alterations of mineralized matrix by lead exposure in osteoblast (MC3T3-E1) culture
Author
Paisrisarn P, Tepaamorndech S, Khongkow M, Khemthong P, Kasamechonchung P, Klysubun W, Wutikhun T, Huang LP, Chantarasakha K, Boonrungsiman S
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Nanotechnology Center (NANOTEC); National Science & Technology Development Agency - Thailand; National Science & Technology Development Agency - Thailand; United States Department of Agriculture (USDA)
Type
Article
Source Title
TOXICOLOGY LETTERS
ISSN
0378-4274
Year
2018
Volume
299
Page
172-181
Open Access
Bronze
Publisher
ELSEVIER IRELAND LTD
DOI
10.1016/j.toxlet.2018.10.008
Format
Abstract
The present study investigated the effect of lead (Pb) on bone ultrastructure and chemistry using an in vitro bone model. MC3T3-E1 preosteoblasts were differentiated and treated with lead acetate at 0.4, 2, 10, and 50 mu M. No abnormalities in either cell growth or bone nodule formation were observed with the treated dose of lead acetate. However, Pb treatments could significantly increase Pb accumulation in differentiated osteoblast cultures and upregulate expression of Divalent metal transporter 1 (Dmt1) in a dose dependent manner. Pb treatments also altered the expression of osteogenic genes, including secreted phosphoprotein 1, osteocalcin, type I collagen, and osteoprotegerin. Moreover, in mineralized osteoblast cultures, Pb was found to be mainly deposited as Pb salts and oxides, respectively. Ultrastructure analysis revealed Pb localizing with calcium and phosphorus in the mineralized matrix. In mineralizing osteoblast cells, Pb was found in the intracellular calcified vesicles which is one of the bone mineralization mechanisms. Pb was also present in mineral deposits with various shapes and sizes, such as small and large globular or needle-like mineral deposits representing early to mature stages of mineral deposits. Furthermore, Pb was found more in the globular deposits than the needle shaped mineral crystals. Taken together, our observations revealed how Pb incorporates into bone tissue, and showed a close association with bone apatite.
Keyword
in vitro bone formation | lead | MC3T3-E1 | mineralization | osteoblast
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
NANOTEC internal grant [P 1651899]; United States Department of Agriculture, Agricultural Research Service [5306-51000-004-00D]
License
Copyright
Rights
Elesvier B.V
Publication Source
WOS