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Andrographolide and Its 14-Aryloxy Analogues Inhibit Zika and Dengue Virus Infection
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Document Title
Andrographolide and Its 14-Aryloxy Analogues Inhibit Zika and Dengue Virus Infection
Author
Li F., Khanom W., Sun X., Paemanee A., Roytrakul S., Wang D., Smith D.R., Zhou G.-C.
Name from Authors Collection
Affiliations
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211816, China; Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon Sai 4, Salaya, Thailand; Proteomics Research Laboratory, Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park ,Phahonyothin Road, Khlong Luang, Thailand
Type
Article
Source Title
Molecules (Basel, Switzerland)
ISSN
14203049
Year
2020
Volume
25
Issue
21
Open Access
Gold, Green
Publisher
NLM (Medline)
DOI
10.3390/molecules25215037
Abstract
Andrographolide is a labdene diterpenoid with potential applications against a number of viruses, including the mosquito-transmitted dengue virus (DENV). In this study, we evaluated the anti-viral activity of three 14-aryloxy analogues (ZAD-1 to ZAD-3) of andrographolide against Zika virus (ZIKV) and DENV. Interestingly, one analogue, ZAD-1, showed better activity against both ZIKV and DENV than the parental andrographolide. A two-dimension (2D) proteomic analysis of human A549 cells treated with ZAD-1 compared to cells treated with andrographolide identified four differentially expressed proteins (heat shock 70 kDa protein 1 (HSPA1A), phosphoglycerate kinase 1 (PGK1), transketolase (TKT) and GTP-binding nuclear protein Ran (Ran)). Western blot analysis confirmed that ZAD-1 treatment downregulated expression of HSPA1A and upregulated expression of PGK1 as compared to andrographolide treatment. These results suggest that 14-aryloxy analogues of andrographolide have the potential for further development as anti-DENV and anti-ZIKV agents.
Keyword
Andrographolide | Dengue virus | HSPA1A | PGK1 | Zika virus
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus