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Assembly of the durian chloroplast genome using long PacBio reads
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Document Title
Assembly of the durian chloroplast genome using long PacBio reads
Author
Shearman J.R., Sonthirod C., Naktang C., Sangsrakru D., Yoocha T., Chatbanyong R., Vorakuldumrongchai S., Chusri O., Tangphatsornruang S., Pootakham W.
Name from Authors Collection
Scopus Author ID
12779115900
Scopus Author ID
56030033100
Affiliations
National Omics Center, National Science and Technology Development Agency, 111 Thailand Science Park, Paholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand; Department of Agriculture, Chantaburi Horticultural Research Center, Chanthaburi, 22110, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2020
Volume
10
Issue
1
Open Access
All Open Access, Gold, Green
Publisher
Nature Research
DOI
10.1038/s41598-020-73549-4
Format
Abstract
We have assembled the complete sequence of the Durio zibethinus chloroplast genome using long PacBio reads. Durian is a valuable commercial tree that produces durian fruit, which is popular in Southeast Asia. The chloroplast genome assembled into a single 143 kb cyclic contig that contained 111 genes. There were 46 short direct repeats (45 to 586 bp) and five short inverted repeats (63 to 169 bp). The long reads that were used for the assembly span the entire chloroplast with > 10 kb overlaps and multiple long reads join the start of the contig to the end of the contig. The durian chloroplast was found to lack the large inverted repeat that is common in chloroplast genomes. An additional 24 durian varieties were sequenced and compared to the assembly and found to also lack the large inverted repeat. There were nine SNPs among the varieties. © 2020, The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency
License
N/A
Rights
N/A
Publication Source
Scopus