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Bacteria-induced Dscam isoforms of the crustacean, Pacifastacus leniusculus
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Document Title
Bacteria-induced Dscam isoforms of the crustacean, Pacifastacus leniusculus
Author
Watthanasurorot A.,Jiravanichpaisal P.,Liu H.,Söderhäll I.,Söderhäll K.
Name from Authors Collection
Affiliations
Department of Comparative Physiology, Uppsala University, Uppsala, Sweden; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand; State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian, China
Type
Article
Source Title
PLoS Pathogens
ISSN
15537366
Year
2011
Volume
7
Issue
6
Open Access
All Open Access, Gold, Green
DOI
10.1371/journal.ppat.1002062
Abstract
The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in the N-terminal half of Ig2 and Ig3 and the complete Ig7 and in the transmembrane domain. The cytoplasmic tail can generate multiple isoforms. PlDscam can generate more than 22,000 different unique isoforms. Bacteria and LPS injection enhanced the expression of PlDscam, but no response in expression occurred after a white spot syndrome virus (WSSV) infection or injection with peptidoglycans. Furthermore, PlDscam silencing did not have any effect on the replication of the WSSV. Bacterial specific isoforms of PlDscam were shown to have a specific binding property to each tested bacteria, E. coli or S. aureus. The bacteria specific isoforms of PlDscam were shown to be associated with bacterial clearance and phagocytosis in crayfish. © 2011 Watthanasurorot et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus