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Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification
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Document Title
Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification
Author
Wanmolee W, Sornlake W, Rattanaphan N, Suwannarangsee S, Laosiripojana N, Champreda V
Name from Authors Collection
Scopus Author ID
24329571800
Affiliations
King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
BMC BIOTECHNOLOGY
Year
2016
Volume
16
Issue
5
Open Access
Green Published, gold
Publisher
BMC
DOI
10.1186/s12896-016-0312-7
Format
Abstract
Background: Efficient hydrolysis of lignocellulosic materials to sugars for conversion to biofuels and chemicals is a key step in biorefinery. Designing an active saccharifying enzyme system with synergy among their components is considered a promising approach. Results: In this study, a lignocellulose-degrading enzyme system of Chaetomium globosum BCC5776 (CG-Cel) was characterized for its activity and proteomic profiles, and synergism with accessory enzymes. The highest cellulase productivity of 0.40 FPU/mL was found for CG-Cel under the optimized submerged fermentation conditions on 1% (w/v) EPFB (empty palm fruit bunch), 2% microcrystalline cellulose (Avicel (R)) and 1% soybean meal (SBM) at 30 degrees C, pH 5.8 for 6 d. CG-Cel worked optimally at 50-60 degrees C in an acidic pH range. Proteomics analysis by LC/MS/MS revealed a complex enzyme system composed of core cellulases and accessory hydrolytic/non-hydrolytic enzymes attacking plant biopolymers. A synergistic enzyme system comprising the CG-Cel, a beta-glucosidase (Novozyme (R) 188) and a hemicellulase Accellerase (R) XY was optimized on saccharification of alkaline-pretreated rice straw by a mixture design approach. Applying a full cubic model, the optimal ratio of ternary enzyme mixture containing CG-Cel: Novozyme (R) 188: Accellerase (R) XY of 44.4:20.6:35.0 showed synergistic enhancement on reducing sugar yield with a glucose releasing efficiency of 256.4 mg/FPU, equivalent to a 2.9 times compared with that from CG-Cel alone. Conclusions: The work showed an approach for developing an active synergistic enzyme system based on the newly characterized C. globosum for lignocellulose saccharification and modification in bio-industries.
Keyword
Cellulase | Chaetomium globosum | lignocellulose | Saccharification | Synergistic action
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency [P-15-50502]; Thailand Research Fund; Petchra Pra Jom Klao Ph.D. Research Scholarship, King Mongkut's University of Technology Thonburi; Joint Graduate School of Energy and Environment
License
CC BY
Rights
Authors
Publication Source
WOS