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Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages
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Document Title
Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages
Author
Kaewseekhao B., Roytrakul S., Yingchutrakul Y., Laohaviroj M., Salao K., Faksri K.
Name from Authors Collection
Affiliations
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases (RCEID), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand
Type
Article
Source Title
PeerJ
ISSN
21678359
Year
2021
Volume
9
Open Access
Gold, Green
Publisher
PeerJ Inc.
DOI
10.7717/peerj.11565
Abstract
Background. Differences in immune responses against different lineages of Mycobac- terium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune responses were also investigated during infection and when the bacilli had been eliminated from the immune cells. Methods. Human primary leukocytes were infected with strains representing three lineages of Mtb (East-Asian, Indo-Oceanic and Euro-American). Label-free GeLC MS/MS proteomic analysis of secretomes was performed. The response of each immune-cell type was compared with the appropriate interactome database for each. Results. The expression pattern of proteins secreted by Mtb-infected leukocytes differed among Mtb lineages. The ancestral lineage (IO lineage) had a greater ability to activate MMP14 (associated with leukocyte migration) than did the more recent lineages (EA and EuA). During infection, proteins secreted by macrophages, dendritic cells, neutrophils and B-cells were associated with cell proliferation. Following clearance of Mtb, proteins associated with interferon signaling were found in macrophages, dendritic cells and neutrophils: Proteins associated with antigen processing were found in B-cells and regulatory T-cells. Expression of immune response-related proteins from many immune-cell types might be suppressed by Mtb infection. Our study has provided a better insight into the host-pathogen interaction and immune response against different Mtb lineages. © 2021 PeerJ Inc.. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Khon Kaen University
License
CC BY
Rights
Author
Publication Source
Scopus