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Characterization of dengue virus entry into HepG2 cells
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Metadata
Document Title
Characterization of dengue virus entry into HepG2 cells
Author
Suksanpaisan L., Susantad T., Smith D.R.
Name from Authors Collection
Affiliations
Molecular Pathology Laboratory, Salaya Campus, Mahidol University, 25/25 Phuttamontol Sai 4, Salaya, Nakorn Pathom, 73170, Thailand; Molecular Medicine Program, Mayo Clinic College of Medicine, Guggenheim 18, 200 First street SW, Rochester, MN 55905, United States; National Science and Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand
Type
Article
Source Title
Journal of Biomedical Science
ISSN
10217770
Year
2009
Volume
16
Issue
1
Open Access
Gold, Green
DOI
10.1186/1423-0127-16-17
Abstract
Background. Despite infections by the dengue virus being a significant problem in tropical and sub-tropical countries, the mechanism by which the dengue virus enters into mammalian cells remains poorly described. Methods. A combination of biochemical inhibition, dominant negative transfection of Eps15 and siRNA mediated gene silencing was used to explore the entry mechanism of dengue into HepG2 cells. Results. Results were consistent with entry via multiple pathways, specifically via clathrin coated pit mediated endocytosis and macropinocytosis, with clathrin mediated endocytosis being the predominant pathway. Conclusion. We propose that entry of the dengue virus to mammalian cells can occur by multiple pathways, and this opens the possibility of the virus being directed to multiple cellular compartments. This would have significant implications in understanding the interaction of the dengue virus with the host cell machinery.
Funding Sponsor
Mahidol University; Thailand Research Fund
License
CC BY
Rights
Author
Publication Source
Scopus