-
Characterization of yeast and bacterial type strains with food and agricultural applications by MALDI-TOF mass spectrometry biotyping
- Back
Document Title
Characterization of yeast and bacterial type strains with food and agricultural applications by MALDI-TOF mass spectrometry biotyping
Author
Harnpicharnchai P., Jaresitthikunchai J., Seesang M., Jindamorakot S., Tanapongpipat S., Ingsriswang S.
Name from Authors Collection
Scopus Author ID
57218353498
Scopus Author ID
6602764100
Affiliations
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Phahonyothin Road., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Microbiology and Biotechnology Letters
ISSN
1598642X
Year
2020
Volume
48
Issue
2
Page
138-147
Open Access
All Open Access, Bronze
Publisher
Korean Society for Microbiology and Biotechnology
DOI
10.4014/mbl.1910.10008
Format
Abstract
Various microorganisms play important roles in food fermentation, food spoilage, and agriculture. In this study, the biotype of 54 yeast and bacterial strains having high potential for utilization in food and agriculture, including Candida spp., Lactobacillus spp., and Acetobacter spp., were characterized by matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS). This characterization using a fast and robust method provides much-needed information on the selected microorganisms and will facilitate effective usage of these strains in various applications. Importantly, the unique protein profile of each microbial species obtained from this study was used to create a database of fingerprints from these species. The database was validated using microbial strains of the same species by comparing the mass spectra with the created database through pattern matching. The created reference database provides crucial information and is useful for further utilization of a large number of valuable microorganisms relevant to food and agriculture. © 2020, The Korean Society for Microbiology and Biotechnology
Keyword
Agriculture | Biotyping | Food | MALDI-TOF Mass Spectrometry | Microorganisms
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; National Center for Genetic Engineering and Biotechnology
License
CC BY
Rights
N/A
Publication Source
Scopus