• Chitooligosaccharide from Pacific White Shrimp Shell Chitosan Ameliorates Inflammation and Oxidative Stress via NF-?B Erk1/2 Akt and Nrf2/HO-1 Pathways in LPS-Induced RAW264.7 Macrophage Cells

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Document Title

Chitooligosaccharide from Pacific White Shrimp Shell Chitosan Ameliorates Inflammation and Oxidative Stress via NF-?B Erk1/2 Akt and Nrf2/HO-1 Pathways in LPS-Induced RAW264.7 Macrophage Cells

Author

Chotphruethipong L. Chanvorachote P. Reudhabibadh R. Singh A. Benjakul S. Roytrakul S. Hutamekalin P.

Affiliations

Department of Food Science Faculty of Science Burapha University Mueang Chonburi Chonburi 20131 Thailand; Department of Pharmacology and Physiology Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok 10330 Thailand; Center of Excellence in Cancer Cell and Molecular Biology Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok 10330 Thailand; Faculty of Science and Technology Hatyai University Hat Yai 90110 Thailand; International Center of Excellence in Seafood Science and Innovation Faculty of Agro-Industry Prince of Songkla University Hat Yai 90110 Thailand; Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Pathum Thani 12120 Thailand; Division of Health and Applied Sciences Faculty of Science Prince of Songkla University Hat Yai 90110 Thailand

Type

Article

Source Title

Foods

ISSN

23048158

Year

2023

Volume

12

Issue

14

Open Access

All Open Access Gold Green

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

DOI

10.3390/foods12142740

Abstract

Chitooligosaccharide (COS) found in both insects and marine sources has several bioactivities such as anti-inflammation and antioxidant activities. However the mechanism of shrimp shell COS on retardation of inflammatory and antioxidant effects is limited. Therefore the aim of this study is to examine the mechanism of the aforementioned activities of COS in LPS-activated RAW264.7 macrophage cells. COS significantly improved cell viability in LPS-activated cells. COS at the level of 500 ?g/mL could reduce the TNF-? NO and IL-6 generations in LPS-activated cells (p < 0.05). Furthermore COS could reduce ROS formation NF-?B overactivation phosphorylation of Erk1/2 and Akt and Nrf2/HO-1 in LPS-exposed cells. These results indicate that COS manifests anti-inflammatory activity and antioxidant action via NF-?B Erk1/2 Akt and Nrf2/HO-1 signaling with an increasing relevance for inflammatory disorders. ? 2023 by the authors.

Industrial Classification

N/A

Knowledge Taxonomy Level 1

Biological Sciences

Knowledge Taxonomy Level 2

Biology and biochemistry

Knowledge Taxonomy Level 3

Biochemistry

License

CC BY

Rights

Authors

Publication Source

WOS

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