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Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae
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Document Title
Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae
Author
Promdonkoy B, Promdonkoy P, Panyim S
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University; Mahidol University
Type
Article
Source Title
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097
Year
2005
Volume
252
Open Access
Bronze
Publisher
OXFORD UNIV PRESS
DOI
10.1016/j.femsle.2005.08.038
Format
Abstract
Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC50 = 140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC50 values of 350 and 250 ng ml(-1), respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti and C. quinquefasciatus larvae (LC50 = 7 and 20 ng ml(-1), respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keyword
Bacillus thuringiensis | Co-expression | Crystal toxin | Endotoxin | Mosquito | Synergism
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
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Copyright
Rights
Publisher
Publication Source
WOS